D02. Linalool Exerted a Neuroprotective Activity against Corticosterone-induced Apoptosis of PC12 cells
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Abstract
Introduction: Linalool is an active compound found in many essential oils having sedative, anxiolytic and analgesic properties. Therefore, these essential oils have been usually used in aromatherapy to reduce stress. However, the mechanism underlying anti-stress of linalool has not been investigated.
Objective: To investigate the mechanism of neuroprotective action of linalool on corticosterone-induced neurotoxicity in PC12 cells.
Methods: PC12 cells were treated with 650 μM of corticosterone in the absence or presence of linalool or fluoxetine for 24 hours. PC12 was treated with corticosterone 650 μM and difference concentration of linalool (100, 200, 300, 400, 500, 600 and 700 μM) for 24 hours. Methyl thiazolyl tetrazolium (MTT) assay and lactate dehydrogenase (LDH) detection were investigated to confirm the neuroprotective effect of linalool against cell damage caused by corticosterone. Then, the intracellular Ca2+ content was measured by fluorescent labeling.
Results: Linalool 100 and 200 μM significantly increased cell viability compared with the group treated with corticosterone alone (p<0.05). Linalool 100, 200, 300 and 400 μM significantly (p<0.05) reduced LDH release. Linalool (100, 200, 300, 400, 500 and 600 μM) tend to revere the increased intracellular Ca2+produced by corticosterone.
Conclusion: These findings indicated that linalool exerted a neuroprotective activity against corticosterone-induced neuronal apoptosis in PC12 cells. The mechanism underlying this action may be partly due to the attenuation of intracellular Ca2+ overloading under stress conditions. However, further studies need to investigate the precise cellular and molecular mechanisms underlying this neuroprotective activity of linalool.
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