C29. Effects of Electroacupuncture on FUNdC1-LC3 Signaling Pathway in Rats Cerebral Ischemia-reperfusion Injury
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Abstract
Objective: To observe the effect of electrotherapy on the FundC1-LC3 signaling pathway in cerebral ischemia reperfusion injury (CIRI) rats and to explore the mechanism of its intervention on CIRI from the perspective of mitochondrial autophagy.
Methods: 24 SD rats were randomly divided into sham operation group, model group and electroacupuncture group. In the electroacupuncture group, electroacupuncture treatment was started 24h after modeling for 30min and lasted for 3 days. The neurological function of the rats was scored before sampling. TTC staining was used to calculate the volume of cerebral ischemia infarction in rats. Western blot was used to detect LC3B and FUNDC1 protein expression in cortical region.
Results: Compared with the sham operation group, the neurological function score of the model group was significantly higher, and the neurological function deficit was serious. LC3B protein expression was increased in cortex, and the differences were statistically significant. Compared with the model group, the neurological function score of the electroacupuncture group was significantly lower, and the neurological function defect was improved. LC3B and FUNDC1 protein expressions were increased in cortical area, and the differences were statistically significant.
Conclusion: Electroacupuncture may promote the FUNDC1-LC3 signaling pathway to up-regulate mitochondrial autophagy and alleviate neurological impairment in rats, thus playing a neuroprotective role.
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