C27. Study on the mechanism of exercise therapy in promoting neural stem cell mobilization after stroke
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Abstract
Introduction: Stroke has become the third leading cause of death in humans, most of which are ischemic strokes. Exercise therapy is currently very popular and loved by most people. Studies have found that exercise training can promote neurogenesis, angiogenesis, synaptic plasticity, and neural stem cell proliferation and differentiation after cerebral ischemia. The Wnt/β-catenin signaling pathway regulates cell cycle and neurogenesis.
Objective: To observe the mobilization of endogenous neural stem cells by exercise therapy in rats with cerebral ischemia and its mechanism.
Methods: Thirty SPF adult SD rats were selected, 8 rats were randomly selected as the sham operation group (sham), and the remaining rats were constructed as a middle cerebral artery occlusion (MCAO) model, and then the successfully modeled rats were randomly divided into the model group (MCAO) and exercise group (RUN), exercise group was given exercise treatment 24 hours after MCAO operation, once a day for 9 consecutive days, and model group and sham operation group were not treated. The neurological deficits of the rats were detected by behavior, the volume of cerebral infarction was detected by TTC staining, and the expressions of DCX and Wnt/β-catenin pathway-related proteins wnt3a and β-catenin in the infarcted cortex were detected by western blot.
Results: Compared with the sham operation group, the mNSS score of the model group was significantly higher (P<0.001), and compared with the model group, the mNSS score of the exercise group was lower (P<0.001). The Fault score was lower (P<0.001), and compared with the model group, the Foot-Fault score of the exercise group was higher (P<0.01); TTC staining showed that the cerebral infarction volume in the sham operation group was 0. The volume of cerebral infarction in the group increased significantly (P<0.001). Compared with the model group, the volume of cerebral infarction in the exercise group was reduced, but the difference was not statistically significant. Western blot showed that compared with the sham operation group, the protein expressions of wnt3a（P<0.01）and β-catenin(P<0.05) in the model group were lower, and compared with the model group, the protein expressions of DCX (P<0.05) and wnt3a (P<0.01) in the exercise group were higher.
Conclusion: Exercise therapy may promote the mobilization of endogenous neural stem cells in rats with cerebral ischemia by activating wnt/β-catenin.
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