C25. Molecular Characterization of the Full-length Bipartite Begomovirus Causing Pepper Yellow Leaf Curl Disease, a Common Cause of Crop Damage in Northeast and Central Thailand
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Abstract
Introduction: The incidence of the yellow leaf curl disease of pepper is an important and serious threat for the production of pepper in Thailand. The disease is caused by whitefly-transmitted begomoviruses which can lead to severe economic losses of the crop.
Objective: To determine and describe the complete sequences of begomovirus important species isolated from field infected pepper plants
Methods: In this study, six diseased samples of the Capsicum anuum plants (showing yellow mosaic and leaf curling symptom) were collected from outbreak areas during a survey in 2016 – 2019 and used to extract total DNA. The full-length viral genomes were amplified by rolling circle amplification technology (RCA), cloned and sequenced bi-directionally. The generated sequences were then assembled and characterized including their phylogenetic relationships with other selected begomoviruses.
Results: The sequences of the DNA-A genome from all six isolates showed the highest identity (97%) with the isolates of Pepper yellow leaf curl Thailand virus (PepYLCTHV). For the DNA-B component, it showed the closest cluster with those of PepYLCTHV (90% similarity).
Conclusion: Based on the literature review and current evidence, this is the report confirming the presence of the PepYLCTHV bipartite begomovirus from Thailand.
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