A24. Antimicrobial Activities of Streblus Asper Leaves Extract in Thailand

Abstract

Introduction: Streblus asper, or toothbrush tree, has been traditionally used as a medicinal plant for toothache and dental caries treatments.

Objective: This study aimed to investigate antimicrobial activities of S. asper leaves ethanolic extracts in Thailand.

Methods: S. asper leaves were washed, dried and ground to powdered, then exhaustively extracted with 95% ethanol using Soxhlet extraction. Antimicrobial activity including determined zone of inhibition, determination of minimum inhibitory concentration (MIC), determination of minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were determined, as according to Clinical & Laboratory Standards Institute guidelines. The extract was done against 10 tested microorganisms including, Staphylococcus aureus, Bacillus cereus, Streptococcus mutan, Enterobacter aerogenes, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans and Candida glabrata.

Results: S. asper leaves extract at 200 mg/ml showed an inhibition zone against tested microorganisms, except Pseudomonas aeruginosa, ranging from 7.00 to 11.00 mm. The leaves extract had inhibitory and microbicidal capacity at concentration of            ≥ 2,000 µg/ml, except Staphylococcus aureus and Streptococcus mutan had good inhibitory and microbicidal capacity at concentration of 125 and 250 µg/ml, respectively.

Conclusion: S. asper leaves extract had good activities on oral pathogen bacteria, its leaves extract could be beneficial as an oral hygiene product.