Quality Evaluation and Pectolinarigenin Contents Analysis of Harak Remedy in Thailand
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Abstract
Introduction: Harak remedy is a Thai traditional medicine for anti-pyretic treatment. Some researchers reported that crude drugs and capsules of Harak remedy, which were distributed throughout Thailand, have been adulterated with the upper ground parts. Moreover, there is no recent report of quality control of the Harak products after marketed. Objective: Thus, aim of our research was to investigate the quality of marketed Harak capsules following requirements of Thai Herbal Pharmacopoeia (THP). Methods: The hierarchical cluster analysis (HCA) and principal component analysis (PCA) reported the similarity of samples to authentic Harak. The 18 samples were purchased from 6 regions of Thailand, 3 samples in each region (HR01-HR18). The authentic plants were collected from Surin province and provided as capsule drug (HR19). In addition, the powder of authentic remedy was extracted by aqueous and ethanol then the chemical constituents were analyzed by GC-MS. Results: As the result of standard specifications, 15 samples were standard medicines (83.33%) while 3 samples had high levels of total aerobic bacteria, total yeast, and molds. The chemical fingerprint and quantification of pectolinarigenin of 19 samples were investigated by TLC and RP-HPLC. The pectolinarigenin content of HR19 was 25.3±0.31 mg/g drug powder and the most correlated with were 3 samples as HR08, HR10, and HR16. Conclusion: According to HCA and PCA, most of the samples showed similar data patterns except HR17. The result provides essential information for identification of the Harak remedy for the purpose of quality control.
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