Toxicity of Papaya (Carica papaya, Linnaeus) Extracts against Larvae of Aedesaegyptiand Culexquinquefasciatus

Abstract

The objectives of this experiment were to evaluate the 24 h-LC₅₀ of maceration of 2 types of papaya (Carica papaya, Linnaeus) leaves in 90% ethyl alcohol against the 3^rd instar larvae of Aedesaegyptiborabora and Culexquinquefasciatus.

The results found that percentage yield of fresh and dried leaves of Khakdum papaya crude extracts, and fresh and dried leaves of Srirajabhat papaya crude extracts were8.892, 7.669, 7.465 and7.122%, respectively. The HPLC detected the amount of saponin in crude extracts of fresh and dried of Khakdum papaya leaves and fresh and dried of Srirajabhat leaves were 386.489, 132.340, 204.827and 93.371mg L^-1, respectively. The 24 h-LC₅₀ of crude extracts of fresh and dried of Khakdum papaya leaves and fresh and dried of Srirajabhat leaves against the 3^rd-instarlarvae of Aedesaegypti (borabora) were 0.00975, 0.00487, 0.01110, and 0.00980 mg L^-1, respectively.Moreover,24 h-LC₅₀ of crude extracts of fresh and dried of Khakdum papaya leaves and fresh and dried of Srirajabhat leaves against the 3^rd-instar larvae of Culexquinquefasciatus were 0.01375, 0.00928, 0.01672, and 0.01228 mg L^-1, respectively.Stereomicroscope was use for descript details of dead larvae, their abdomens were decayed and siphons were swarthy.

In conclusion, the 7-days maceration of dried of Khakdum papaya leaves in 90% ethyl alcoholwould be applied to use asAedes and Culexlarvicide in the next further.