Organophosphate and carbamate insecticide susceptibility in genetically pyrethroid-resistant Aedes aegypti mosquitoes from a dengue risk area in Phitsanulok province
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Abstract
The purpose of this study was to investing the susceptibility of genetically pyrethroid-resistant Ae. aegypti the organophosphate and carbamate insecticide in a dengue risk area in Phitsanulok province. The mosquitoes were tested for susceptibility to individual 16 insecticides status using the WHO procedure. PCR and DNA sequencing were conducted to detect mutation in the para gene in pyrethroid-resistant Ae. aegypti.
The results showed that the mosquitoes were completely susceptible to fenitrothion, malathion, and fenobucarb with mean mortality rates of 100% after 24 h. However, the mosquitoe were resistant to pirimiphos-methyl, propoxur, and 10 pyrethroid insecticides with the mean mortality rates ranging from 3.00±2.00% and 87.00±5.03%. The mortality rates of mosquitoes were significantly different among insecticides (p<0.05). In addition, a mutation in the para gene at position V1016G was detected with a mutation frequency mean of 0.52. Suggesting that organophosphates and carbamates could be alternative insecticides to pyrethroids for controlling genetically pyrethroid-resistant Ae. aegypti and preventing Ae. aegypti-borne diseases.
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ข้อลิขสิทธิ์วารสาร
บทความหรือข้อคิดเห็นใดๆ ที่ปรากฏในวารสารวิชาการป้องกันควบคุมโรค สคร. 2 พิษณุโลก เป็นวรรณกรรมของผู้เขียน กองบรรณาธิการวิชาการ และ สำนักงานป้องกันควบคุมโรคที่ 2 จังหวัดพิษณุโลกไม่จำเป็นต้องเห็นพ้องด้วยทั้งหมดหรือร่วมรับผืิดชอบใดๆ
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