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Objective:This study aimed to assess the antimicrobial efficacy of seed extractderived from Bouea macrophyllaGriffith (Maprang wan)grown in Northern Thailand against Entercoccus faecalis,Streptococcus gordoniiandCandida albicans inplanktonic and multispecies biofilm.
Materials and methods:Agar diffusion and broth microdilution methods were performed to investigate the antimicrobial activities of ethyl acetate extract of Maprang wan seed against planktonic E. faecalis,S. gordoniiand C. albicans.Growth curves of E. faecalis,S. gordoniiand C. albicansin 1:1:1and1:1:0.1ratio, respectively) were performed to measure the appropriate ratio of the species within biofilm. Multispecies of E. faecalis,S. gordoniiand C. albicansbiofilms were grown in RPMI for 48 hours at 37°C. Following this, the biofilms were exposed to 25 mg/mL of Maprang seed extracts. 0.02 mg/mL chlorhexidine was used as the positive control and RPMI as the negative control. After exposure, time-kill assay was conducted to evaluate time-point of the extract which showed bactericidal and fungicidal effects. The colony forming unit (CFU) data were analysed with one-way ANOVAand Dunnett's test (p<0.05).
Results:Maprang seed extract demonstrated the antimicrobial activity against planktonic and multispecies E. faecalis,S. gordoniiand C. albicans. The time kill assay showed a time dependent action of Maprang seed extract, itdemonstrated significant reduction of viable organisms at the time periods.
Conclusions:Maprang seed extract had antibiofilm property against multispecies E. faecalis,S. gordoniiand C. albicansbiofilms. The antimicrobial activity of the extract was comparable to chlorhexidine. Maprang seed extract has potential as a novel antimicrobial agent and could be used to treat superficial infections or as aroot canal medicament.
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