Forensic Identification by Polymerase Chain Reaction of DNA Extracted from Primary Teeth with Dental Caries

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Published: Apr 20, 2021
Keywords:
deciduous teeth polymerase chain reaction microsatellite DNA

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Rujee Tonleuang
Master's Student in Forensic Science,Graduate School, Chiang Mai University, Thailand
Tanin Bhoopat
Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Thailand
Papimon Chompu-Inwai
Department of Orthodontics and Pediatric Dentistry, Faculty of Dentistry, Chiang Mai University, Thailand
Anak Iamaroon
Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Thailand

Abstract

Objective: The aim of this research is to determine the effect of restoration time of deciduous teeth on human identification efficiency using microsatellite DNA


Methods: Using human genomic DNAs were isolated from deciduous molar in Thai children (n=26) under 12 years and were compared with DNA from the blood in sterile gauze pads. All samples were divided into 2 groups of 13 teeth, which were kept at room temperature for a period of 1 day and 1 month, respectively. Tooth DNAs were extracted using QIAamp® DNA Investigator and then microsatellite DNA on D3S1358, D5S818 and D16S539 loci were amplified using polymerase chain reaction.


Results: DNA could be detected from 2 groups. Microsatellite DNA on D3S1358, D5S818 and D16S539 loci showed the same results as the blood samples.


Conclusions: Deciduous teeth stored at room temperature for one month had no affected on human identification efficiency using microsatellite DNA.

Article Details

How to Cite
Tonleuang, R. ., Bhoopat, T. ., Chompu-Inwai, P. ., & Iamaroon, A. (2021). Forensic Identification by Polymerase Chain Reaction of DNA Extracted from Primary Teeth with Dental Caries. Chiang Mai Dental Journal, 42(1), 139–149. Retrieved from https://he01.tci-thaijo.org/index.php/cmdj/article/view/248954
Issue
Vol. 42 No. 1 (2021): January-April
Section
Original article

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