Biologic effects of pulp capping material containing fluocinolone acetonide on human dental pulp cells

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Phumisak Louwakul
Veera Lertchirakarn
Tadkamol Krongbaramee

Abstract

The aims of this study were to test some biological properties of pulp capping material containing fluocinolone acetonide (PCFA). The PCFA is hard setting calcium hydroxide cement containing 50 mmol/L of fluocinolone acetonide (FA). Cytotoxicity and cell proliferation were evaluated by MTT assay. RT-PCR and Western blotting were used to study the effects of PCFA on RNA (dentin sialophosphoprotein: DSPP) and protein (dentin sialoprotein: DSP) synthesis. Anti-inflammatory effect of PCFA was determined by analysis of cyclooxygenase 2 (COX-2) expression. Dycal® was used as control. The results suggested that the diluted conditioned media from PCFA slightly increased cell proliferation, significantly increased DSPP expression and decreased COX-2 expression (p < .05). In term of biologic properties, the PCFA may promote cell proliferation, mineralization and decrease pre-existing inflammation in human dental pulp cells. It may be considered as an alternative pulp capping material in the treatment of inflamed dental pulp.

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How to Cite
Louwakul, P., Lertchirakarn, V., & Krongbaramee, T. (2021). Biologic effects of pulp capping material containing fluocinolone acetonide on human dental pulp cells. Chiang Mai Dental Journal, 42(3), 85–94. Retrieved from https://he01.tci-thaijo.org/index.php/cmdj/article/view/248859
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Original article

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