The Comparison of Degradation Time, Weight Loss and Surface Fibrin Structure Between Gauze-Compression and Heat-Compression Platelet-Rich Fibrin Membrane
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Abstract
Objective: To evaluate the effect of heat-compression to platelet-rich fibrin (PRF) membrane in degradation time, weight loss and to examine surface fibrin structure using scanning electron microscope (SEM).
Methods: Sixty PRF membranes that were prepared from human blood (ten healthy volunteers). Samples were compressed at various temperatures and were arranged into six groups: control (room temperature), 60, 70, 80, 90 and 100°C. Nine of the ten samples from each group were evaluated for their degradation time and weight loss. One remaining sample was examined the surface fibrin structure using SEM.
Results: The 90 and 100°C groups had significantly different degradation times compared to those in the control, 60 and 70°C groups (p<0.05). There was no significant difference in the mean weight percentage among the six groups on each day (p<0.05). But when compared among the days in the same group, the 90 and 100°C groups were significantly different in the early phase to 11 and 6 days, respectively. The surface fibrin structure in the 90 and 100°C groups showed the least interfibrous space and lowest porosity.
Conclusion: The 90 and 100°C groups had significantly longer degradation times and delayed the early stage of degradation. It might be applied in surgical operations that need PRF membrane stability in the early phase.
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Schropp L, Wenzel A, Kostopoulos L, Karring T. Bone healing and soft tissue contour changes following single-tooth extraction: a clinical and radiographic 12-month prospective study. Int J Periodontics Restorative Dent 2003; 23(4): 313-323.
Del Fabbro M, Bortolin M, Taschieri S. Is autologous platelet concentrate beneficial for post-extraction socket healing? A systematic review. Int J Oral Maxillofac Surg 2011; 40: 891-900.
Iasella JM, Greenwell H, Miller RL, et al. Ridge preservation with freeze-dried bone allograft and a collagen membrane compared to extraction alone for implant site development: a clinical and histologic study in humans. J Periodontol 2003; 74(7): 990-999.
Cardaropoli D, Tamagnone L, Roffredo A, Gaveglio L, Cadaropoli G. Socket preservation using bovine bone mineral and collagen membrane: a randomized controlled clinical trial with histologic analysis. Int J Periodontics Restorative Dent 2012; 32(4): 421-430.
Lekovic V, Camargo PM, Klokkevold PR, et al. Preservation of alveolar bone in extraction sockets using bioabsorbable membranes. J Periodontol 1998; 69(9): 1044-1049.
Bottino MC, Thomas V, Schmidt G, et al. Recent advances in the development of GTR/GBR membranes for periodontal regeneration--a materials perspective. Dent Mater 2012; 28(7): 703-721.
Simonpieri A, Del Corso M, Sammartino G, Dohan Ehrenfest DM. The relevance of Choukroun's platelet-rich fibrin and metronidazole during complex maxillary rehabilitations using bone allograft. Part I: a new grafting protocol. Implant Dent 2009; 18(2): 102-111.
Simonpieri A, Del Corso M, Sammartino G, Dohan Ehrenfest DM. The relevance of Choukroun's platelet-rich fibrin and metronidazole during complex maxillary rehabilitations using bone allograft. Part II: implant surgery, prosthodontics, and survival. Implant Dent 2009; 18(3): 220-229.
Gassling V, Purcz N, Braesen JH, et al. Comparison of two different absorbable membranes for the coverage of lateral osteotomy sites in maxillary sinus augmentation: a preliminary study. J Craniomaxillofac Surg 2013; 41(1): 76-82.
Dapper WR, Shubin NJ. GBR The BACKBONE of implant dentistry: PART 1. Dental Products Report 2006; 40(9): 82-84.
Dapper WR, Shubin NJ. GBR: The BACKBONE of implant dentistry: PART 2. Dental Products Report 2006 ;40(10): 124-125.
Hurley LA, Stinchfield FE, Bassett CAL, Lyon WH. The role of soft tissues in Osteogenesis: an experimental study of canine spine fusions. J Bone Joint Surg Am 1959; 41(7): 1243-1266.
Karring T. Regenerative periodontal therapy. J Int Acad Periodontol 2000; 2(4): 101-109.
Aurer A, Jorgic-Srdjak K. Membranes for periodontal regeneration. Acta Stomatol Croat 2005; 39(1): 107-112.
Hartshorne J, Gluckman H. A comprehensive clinical review of Platelet Rich Fibrin (PRF) and its role in promoting tissue healing and regeneration in dentistry. Part 1: definition, development, biological characteristics and function. Int Dent 2016; 6: 14-24.
Naik B, Karunakar P, Jayadev M, Marshal VR. Role of Platelet rich fibrin in wound healing: a critical review. J Conserv Dent 2013; 16(4): 284-293.
Choukroun J, Diss A, Simonpieri A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101: E56-60.
Eshghpour M, Dastmalchi P, Nekooei AH, Nejat A. Effect of platelet-rich fibrin on frequency of alveolar osteitis following mandibular third molar surgery: a double-blinded randomized clinical trial. J Oral Maxillofac Surg 2014; 72(8): 1463-1467.
Bölükbaşı N, Yeniyol S, Tekkesin MS, Altunatmaz K. The use of platelet-rich fibrin in combination with biphasic calcium phosphate in the treatment of bone defects: a histologic and histomorphometric study. Curr Ther Res Clin Exp 2013; 75: 15-21.
Miron RJ, Fujioka-Kobayashi M, Hernandez M, et al. Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry? Clin Oral Investig 2017; 21(8): 2619-2627.
Gassling V, Douglas T, Warnke PH, Açil Y, Wiltfang J, Becker ST. Platelet‐rich fibrin membranes as scaffolds for periosteal tissue engineering. Clin Oral Implants Res 2010; 21(5): 543-549.
Del Corso M, Vervelle A, Simonpieri A, et al. Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 1: Periodontal and dentoalveolar surgery. Curr Pharm Biotechnol 2012; 13(7): 1207-1230.
Aroca S, Keglevich T, Barbieri B, Gera I, Etienne D. Clinical evaluation of a modified coronally advanced flap alone or in combination with a platelet-rich fibrin membrane for the treatment of adjacent multiple gingival recessions: a 6-month study. J Periodontol 2009; 80(2): 244-252.
Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101(3): e37-44.
Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 101(3): e45-50.
Kawase T, Kamiya M, Kobayashi M, et al. The heat-compression technique for the conversion of platelet-rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation. J Biomed Mater Res B Appl Biomater 2015; 103(4): 825-831.
Blumenthal NM. The use of collagen membranes to guide regeneration of new connective tissue attachment in dogs. J Periodontol 1988; 59(12):830-836.
Hartshorne J, Gluckman H. A comprehensive clinical review of Platelet Rich Fibrin (PRF) and its role in promoting tissue healing and regeneration in dentistry. Part II: preparation, optimization, handling and application, benefits and limitations of PRF. Int Dent 2016; 6(5): 34-48.
Walker KA, Markoski LJ, Deeter GA, Spilman GE, Martin DC, JS M. Crosslinking chemistry for high-perfomance polymer networks. Polymer 1994; 35(23): 5012-5017.
Rodella LF, Favero G, Labanca M. Biomaterials in maxillofacial surgery: membranes and grafts. Int J Biomed Sci 2011; 7(2): 81-8.
Wang J, Wang L, Zhou Z, et al. Biodegradable polymer membranes applied in guided bone/tissue regeneration: a review. Polymers 2016; 8(4):115-135.
Veríssimo D, Leitão R, Ribeiro R, et al. Polyanionic collagen membranes for guided tissue regeneration: Effect of progressive glutaraldehyde cross-linking on biocompatibility and degradation. Acta Biomaterialia 2010; 6(10): 4011-4018.
Madurantakam P, Yoganarasimha S, Hasan FK. Characterization of leukocyte-platelet rich fibrin, a novel biomaterial. J Vis Exp 2015; 29(103): 1-8.
Miyata T, Fususe M, Yamane Y, Noishiki Y. A biodegradable antiadhesion collagen membrane with slow release heparin. ASAIO Trans 1988; 34(3): 687-691.
Park JY, Jung IH, Kim YK, et al. Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects. Biomater Res 2015; 19(1): 15-25.
Rothamel D, Schwarz F, Sager M, Herten M, Sculean A, Becker J. Biodegradation of differently cross‐linked collagen membranes: an experimental study in the rat. Clin Oral Implants Res 2005; 16(3): 369-378.
Lee JE, Park JC, Hwang YS, Kim JK, Kim JG, Suh H. Characterization of UV-irradiated dense/porous collagen membranes: morphology, enzymatic degradation, and mechanical properties. Yonsei Med J 2001; 42(2): 172-179.