The Efficacy of a Microplate Spectrophotometer for the Detection of Hydrogen Peroxide from Bleaching Agents
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Abstract
Hydrogen peroxide from bleaching agents can easily penetrate through the tooth structure into the pulpal cavity and may cause damage to pulpal cells. The objective of this study was to evaluate the amount of hydrogen peroxide penetration through the bovine tooth disc into the artificial pulp chamber after bleaching with three different bleaching agents for 1 hour. Bovine tooth discs with a thickness of 3.2 mm were prepared and placed into a modified artificial pulp chamber filled with acetate buffer solution. The specimens were divided into 3 groups and bleached with one of following bleaching agents: group-1, 40% Opalescence™ Boost (hydrogen peroxide); group-2, 20% Opalescence™ PF (carbamide peroxide); and group-3, 10% Opalescence™ PF (carbamide peroxide). The amount of peroxide that penetrated into the artificial pulp chamber was evaluated using either a standard spectrophotometer with cuvette reader or microplate spectrophotometer after 1 hour of bleaching. An increase in the amount of hydrogen peroxide penetration corresponded to an increase in the concentration of bleaching products. The highest amount of hydrogen peroxide penetration was found in the group bleached with 40% hydrogen peroxide (0.0185 mg/ml for standard spectrophotometer, 0.1080 mg/ml for microplate spectrophotometer). The microplate spectrophotometer exhibited greater efficacy for detecting the amount of hydrogen peroxide compared with the standard spectrophotometer.
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