Efficiency of Non-thermal Atmospheric Pressure Plasma on Killing Candida albicans Biofilm
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Abstract
Objectives: To investigate the efficacy of non-thermal atmospheric pressure plasma on killing Candida albicans biofilm.
Methods: Candida albicans (ATCC 10231 and 2 clinical strains) divided into 5 groups. The untreated culture was designated as control group. The rest groups were treated by non-thermal atmospheric pressure plasma for 2, 5, 8 and 10 minutes were designated as test groups 1, 2, 3 and 4 respectively. To investigate the least exposure time on killing yeast cells, quantity of yeast cells was examined by colony counting and MTT assays. In addition, biofilm of all strains of Candida albicans was formed individually on acrylic resin before exposed to non-thermal atmospheric pressure. Viability of the biofilms was investigated by colony counting method.
Results: Candida albicans cultures of ATCC 10231 and 2 clinical strains were significantly reduced after exposed to non-thermal atmospheric pressure plasma for 8 minutes. (p<0.05) By colony counting, the growth of the reference and clinical strains were inhibited by 92.30, 88.35 and 88.92 % respectively. Contradictorily, by MTT assay method, the plasma exposure reduced the growth of Candida albicans cells by 26-51% approximately. Additionally, non-thermal atmospheric pressure plasma exposure for 8 minutes reduced Candida albicans biofilms formed on acrylic resin greatly (2-log10 to 4-log10 reduction). (p<0.05)
Conclusion: Non-thermal atmospheric pressure plasma exposure for 8 minutes have demonstrated efficiency in killing Candida albicans biofilms established on acrylic resin.
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References
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