Reference Intervals for serum of 50% hemolytic complement (CH50) activity in Thai blood donors using the liposome immunoassay

Authors

  • Kanthika Bootdee Department of Microbiology, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand
  • Sompong Boonhai Antiserum and standard cell preparation section, National Blood Centre, The Thai Red Cross Society, Bangkok, Thailand
  • Krittraporn Deesin Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Vararat Putthong Department of Microbiology, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand
  • Yadah Kaewopas Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Kanthaporn Dityen Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Niramol Thammacharoenrach Department of Microbiology, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand
  • Asada Leelahavanichkul Department of Medicine, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

Keywords:

Reference ranges, CH50, complement, Thai blood donor, liposome immunoassay

Abstract

Background: For a proper clinical interpretation, it is advised to confirm reference ranges for most of the tests in some countries due to a possible variation from ethnicity.
Objective: This study aimed to define the reference range for complement measurement using the samples from Thai blood donors.
Methods: This study was conducted from January to July 2021 at Chulalongkorn University, Bangkok Thailand. Serum levels of 50% hemolytic complement (CH50) activity,C3 and C4 in 180 donors (17 gif.latex?\pm 61 years old). CH50 was measured using liposome immunoassay, C3 and C4 were based on turbidimetry assay.
Results: The normal values of CH50 in Thai population was 55.86 gif.latex?\pm 9.02 units/ml. Meanwhile, the values of serum C3and C4 were 134.67 gif.latex?\pm 25.63 and 27.20 gif.latex?\pm 8.96 mg/dl, respectively.
Conclusion: The normal serum value of CH50 from the Thai healthy blood donors is 37.83 – 73.89 units/ml. The results of this research can be considered as a reference for Thai population, and may use in the laboratory clinical diagnosis and research.

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Published

2022-02-15

Issue

Vol. 4 No. 1 (2022): January - June 2022

Section

Original article