Characteristics of murine monoclonal anti-H from cell line 5A9 produced by hybridoma technique of the National Blood Center, Thai Red Cross Society
Keywords:
Murine monoclonal antibody, anti-H, MoAbAbstract
Background: H substance is necessary for production of A and B antigens in A and B blood group. However, H antigen deficiency such as Bombay phenotype (Oh), no H antigen presented on red cell surface results in no A or B antigen. People who carry Bombay phenotype can produce natural occurring anti-H in their serum. Thus, Oh recipient can receive only Oh blood transfusion. Anti-H reagent was applied to discriminate Oh from common O blood group.
Objectives: To study the characteristics of murine monoclonal anti-H from cell line 5A9 produced by hybridoma technique of the National Blood Center, Thai Red Cross Society.
Methods: The NBC: anti-H (murine MoAb) produced from cell line 5A9 was tested for potency, specificity and antibody stability compared with 2 murine monoclonal anti-H {Japan: anti-H (murine MoAb), Alba: anti-H (murine MoAb)} and NBC: anti-H (lectin) of the National Blood Center, Thai Red Cross Society.
Results: The specificity of three murine monoclonal anti-H and one of anti-H (lectin) showed the similar results but it was found that the antibody potency of NBC: anti-H (murine MoAb) from cell line 5A9 showed the highest at a titer of 1,024 with a total score of 109.
Conclusion: NBC: anti-H (murine MoAb) from cell line 5A9 of the National Blood Center, Thai Red Cross Society is qualified to be used in the production of murine monoclonal anti-H for the detection of H antigen in both patients and blood donors.
