The results agreement between the CoviQuick v2.0 RTqPCR Kit and AllplexTM 2019-nCoV assay for the detection of SARS-CoV-2 infection


  • Sornsith Jirungda -
  • Tunchanok Prachakul
  • Apisada Kitsanasuwan
  • Suparat Baisungnuen
  • Kreangkri Thaitrakull


The detection of SARS CoV-2 utilizing the AllplexTM 2019-nCoV assay includes RNA extraction steps. A standard RNA extraction is used in the AllplexTM 2019-nCoV assay. The results of the AllplexTM 2019-nCoV assay and the CoviQuick v2.0 RT-qPCR Kit, which use direct specimens in nasopharyngeal and throat swabs, were compared in this study. Seventy-three patient samples were collected, including 28 positive and 45 negative samples. The concordance rate between both tests was 90.4% (66/73) and the inconsistency rate was 9.6% (7/73). The positive correlation was 75.0%, while the negative correlation was 100%. Furthermore, the CoviQuick v2.0 RT-qPCR Kit's correlation in positive samples with cycle threshold (Ct) > 25 was reduced to 68.4%. The CoviQuick v2.0 RT-qPCR Kit showed significantly higher Ct values for the N gene than the AllplexTM 2019-nCoV assay (p = 0.012). These findings imply that the CoviQuick v2.0 RT-qPCR Kit could be used to replace the prior reagent kit in the case of positive samples with Ct < 25 since it is more convenient and reduces turnaround time. If the CoviQuick v2.0 RT-qPCR Kit shows negative results, the procedure must be redone.

Keywords: SARS-CoV-2, COVID-19, real-time RT-PCR, AllplexTM 2019-nCoV assay, CoviQuick v2.0 RT-qPCR Kit


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How to Cite

Jirungda, S., Prachakul, T., Kitsanasuwan, A., Baisungnuen, S., & Thaitrakull, K. (2023). The results agreement between the CoviQuick v2.0 RTqPCR Kit and AllplexTM 2019-nCoV assay for the detection of SARS-CoV-2 infection. Saraburi Hospital Medical Journal, 37(2), 266336/1–10. Retrieved from



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