Microbial Air Contamination in Dental Clinics with Different Ventilation Systems

Main Article Content

Watunyou Khodkaew
Somkiat Luengpailin
Angkana Klungthong

Abstract

The study aimed to compare the Total Viable Aerobic Count (TVACs) before and during dental procedures in two different ventilation systems, the first systems in a Pre-Upgrade Dental Clinic that has separated air conditioners and standing air purifiers, the second systems in a Special Dental Clinic, and a Post-Upgrade Dental Clinic, which have a centralized ventilation and air purifying systems, with the Index of Microbial Air Contamination (IMA).  The data was collected the numbers of bacteria and fungi colony in blood agar (BA) and Sabouraud dextrose agar (SDA). The settle plates were placed 50 cm from a patient’s head, and 1 metre from the wall. Each sampling was placed 1 hour before and during dental procedures, for three days. The Result, in the Special Dental Clinic, the Pre-Upgrade Dental Clinic and the Post-Upgrade Dental Clinic TVACs before and during dental procedures were 71 and 46, 58 and 45, 93 and 15 CFU/dm2/hr, respectively. The Wilcoxon Sign-Rank test showed a statistically significant difference for each setting (p<0.001). Considering three days of data collection, The Kruskal Wallis test indicated that in the Special Dental Clinic (before and during dental procedure) and the Pre-Upgrade Dental Clinic (during procedure), TVACs showed no statistically significant difference. The TVACs showed statistically significant difference in the Pre-Upgrade Dental Clinic (before dental procedure) and the Post-Upgrade Dental Clinic (before and during dental procedure) (p<0.001) Conclusion, the IMA indicated “fair” for the Special Dental Clinic and the Pre-Upgrade Dental Clinic before and during dental procedures. In contrast, the Post-Upgrade Dental Clinic indicated “poor” before dental procedure and “good” in during dental procedure. The TVACs of 2 ventilation systems showed less TVAC during dental procedure than before dental procedure, with a statistically significant.

Article Details

How to Cite
1.
Khodkaew W, Luengpailin S, Klungthong A. Microbial Air Contamination in Dental Clinics with Different Ventilation Systems. Khon Kaen Dent J [Internet]. 2024 Apr. 22 [cited 2024 May 6];27(1):87-96. Available from: https://he01.tci-thaijo.org/index.php/KDJ/article/view/267873
Section
Research Articles
Share |

References

Phelan AL, Katz R, Gostin LO. The Novel Coronavirus Originating in Wuhan, China: Challenges for Global Health Governance. JAMA 2020;323(8):709-10

Meng L, Hua F, Bian Z. Coronavirus disease 2019 (COVID-19): emerging and future challenges for dental and oral medicine. J Dent Res 2020;99(5):481-87

The International Health Policy Program, Thailand (IHPP), Socio-Economical Effects from COVID-19 pandemic in Global and Thailand. Bangkok: Ministry of Public Health; c2019-2023 [cited 2023 Jan 27]. Department of Disease Control; [about 25 screens]. Available from https://ddc.moph.go.th/uploads/publish/ 1177420210915075055.pdf.

Caggiano M, Acerra A, Martina S, Galdi M, D’Ambrosio F. Infection control in dental practice during the COVID-19 pandemic: what is changed. IJERPH. 2023;20(5):3903.

Wang Q, Wang X, Lin H. The role of triage in the prevention and control of COVID-19. ICHE. 2020; 41(7):772-76.

Yadav N, Agrawal B, Maheshwari C. Role of high-efficiency particulate arrestor filters in control of air borne infections in dental clinics. SRM J Res Dent Sci 2015;6(4):240-2.

Pasquarella C, Albertini R, Dall’aglio P, Saccani E, Sansebastiano GE, Signorelli C: Air microbial sampling: the state of the art. Ig. Sanita Pubbl 2008;64(1):79-120.

Pasquarella C, Pitzurra O, Savino A. The index of microbial air contamination. J Hosp Infect 2000;46(4): 241-56

Inyawilert K, BunSyatratchata O, Klangthong A, Kamsa-ard S, Luengpailin S, Rattanathongkom A. Bacterial air contamination inside the dental clinic II and special dental clinic at the dental hospital, faculty of dentistry, khon kaen university. Khon Kaen Dent J 2016;19(2): 107-14

Manarte-Monteiro P, Carvalho A, Pina C, Oliveira H, Manso MC. Air quality assessment during dental practice: Aerosols bacterial counts in an universitary clinic. Rev Port Estomatol Med Dentária e Cir Maxilofac 2013;54(1):2-7

Jain M, Mathur A, Mukhi PU, Ahire M, Pingal C. Qualitative and quantitative analysis of bacterial aerosols in dental clinical settings: Risk exposure towards dentist, auxiliary staff, and patients. J Family Med Prim Care 2020;9(2):1003-008

Dehghani M, Sorooshian A, Nazmara S, Baghani AN, Delikhoon M. Concentration and type of bioaerosols before and after conventional disinfection and sterilization procedures inside hospital operating rooms. Ecotoxicol Environ Saf 2018;164:277-82

Guida M, Gallé F, Di Onofrio V, Nastro RA, Battista M, Liguori G, et al. Environmental microbial contamination in dental setting: a local experience. J Prev Med Hyg 2012;53(4):207-12

Bârlean L, Iancu LS, Minea ML, Dãnilã I, Baciu D. Airborne microbial contamination in dental practices in Iasi, Romania. Ohdmbsc 2010;9(1):16-20

Kedjarune U, Kukiattrakoon B, Yapong B, Chowanadisai S, Leggat PA. Bacterial aerosols in the dental clinic: effect of time, position and type of treatment. Int Dent J 2000;50(2):103-7

Thai Dental council [homepage on the Internet]. Dental safety guide line 2023, [updated 2024; cited 2024 Mar 5]. Available from: https://dentalcouncil.or.th/ upload/ files/1WExCviGHZFpLU4Q0gmSd6MsXzcy DbK.pdf

Hallier C, Williams DW, Potts AJC, Lewis MAO. A pilot study of bioaerosol reduction using an air cleaning system during dental procedures. Br Dent J 2010; 209(8):E14

Samaranayake LP, Fakhruddin KS, Buranawat B, Panduwawala C. The efficacy of bio-aerosol reducing procedures used in dentistry: a systematic review Acta Odontol Scand 2021;79(1):69-80

Maurais MT, Kriese LNJ, Fournier MM, Langevin LL, Mac Leod B, Blier LS, et al. Effectiveness of selected air cleaning devices during dental procedures. Mil Med 2023;188(1-2):e80-85

Lenzer B, Rupprecht M, Hoffmann C, Hoffmann P, Liebers U. Health effects of heating, ventilation and air conditioning on hospital patients: a scoping review. BMC Public Health 2020;20(1):1287.

Qiu Y, Zhou Y, Chang Y, Liang X, Zhang H, Lin X, et al. The effects of ventilation, humidity, and temperature on bacterial growth and bacterial genera distribution. Int J Environ Res Public Health. 2022 Nov 20;19(22):15345.

Holliday R, Allison JR, Currie CC, Edwards DC, Bowes C, Pickering K, et al. Evaluating contaminated dental aerosol and splatter in an open plan clinic environment: Implications for the COVID-19 pandemic. J Dent 2021;105:103565

Obbard, JP, Fang LS. Airborne concentrations of bacteria in a hospital environment in singapore. Water, Air, & Soil Pollution 2003;144(1):333-41.

Hiwar W, King MF, Shuweihdi F, Fletcher LA, Dancer SJ, Noakes CJ. What is the relationship between indoor air quality parameters and airborne microorganisms in hospital environments? A systematic review and meta-analysis. Indoor Air 2021;31(5):1308-322.

Chegini FM, Baghani AN, Hassanvand MS, Sorooshian A, Golbaz S, Bakhtiari R, et al. Indoor and outdoor airborne bacterial and fungal air quality in kindergartens: Seasonal distribution, genera, levels, and factors influencing their concentration. Build Environ [Internet]. 2020;175:106690. Available from: https://doi.org/ 10.1016/j.buildenv.2020.106690

Tolabi Z, Alimohammadi M, Hassanvand MS, Nabizadeh R, Soleimani H, Zarei A. The investigation of type and concentration of bio-aerosols in the air of surgical rooms: A case study in Shariati hospital, Karaj. MethodsX 2019;6:641-50.

Mousavi ES, Kananizadeh N, Martinello RA, Sherman JD. COVID-19 outbreak and hospital air quality: a systematic review of evidence on air filtration and recirculation. Environ Sci Technol 2021;55(7):4134-4147