Effect of Blood Contamination on Dislodgment Resistance of Three Calcium Silicate Cements in Furcation Perforation Models

Main Article Content

Arraya Clawvuthinan
Chinalai Piyachon
Kunlanun Dumrongvute

Abstract

The objective of this study is to compare the dislodgement resistance of three calcium silicate cements in the presence and absence of blood contamination. The study was performed on 48 human permanent molar teeth by creating a furcation perforation in the center of the pulpal floor with a diameter of 1.3 mm and a depth of 2 mm. The samples were randomly divided into two groups: the blood-contaminated and the uncontaminated groups. Each group was divided into three subgroups according to the type of material tested: White ProRoot MTA, Biodentine, and Retro MTA. Each subgroup included eight samples. In the blood-contaminated group, the walls of the perforated area were contaminated with blood before being filled with material, while the uncontaminated group was rinsed with saline. The samples were kept in an incubator at 37°C with 100% relative humidity for seven days before testing. The push-out bond strength was determined with a universal testing machine. Data were analyzed using two-way ANOVA and post-hoc Sidak test (p<0.05). A failure pattern was examined using a dental operating microscope at 25x magnification. The results showed that the dislodgement resistance of Biodentine, in the presence and absence of blood contamination, was significantly higher than White ProRoot MTA and Retro MTA. White ProRoot MTA and Retro MTA showed no significant difference in terms of dislodgement resistance. In the presence of blood contamination, the dislodgement resistance of three materials was significantly lower compared to the absence of blood contamination. Most failure patterns were mixed failures (89.58%). This study concluded that blood contamination reduced the dislodgement resistance of three calcium silicate cements. Biodentine had higher dislodgement resistance than White ProRoot MTA and Retro MTA.

Article Details

How to Cite
1.
Clawvuthinan A, Piyachon C, Dumrongvute K. Effect of Blood Contamination on Dislodgment Resistance of Three Calcium Silicate Cements in Furcation Perforation Models. Khon Kaen Dent J [Internet]. 2023 Sep. 29 [cited 2024 Nov. 20];26(3):27-36. Available from: https://he01.tci-thaijo.org/index.php/KDJ/article/view/256644
Section
Articles
Share |

References

Estrela C, Decurcio DA, Rossi-Fedele G, Silva JA, Guedes OA, Borges Á H. Root perforations: a review of diagnosis, prognosis and materials. Braz Oral Res 2018;32(suppl 1):e73.

Touré B, Faye B, Kane AW, Lo CM, Niang B, Boucher Y. Analysis of reasons for extraction of endodontically treated teeth: a prospective study. J Endod 2011;37(11):1512-5.

Fuss Z, Trope M. Root perforations: classification and treatment choices based on prognostic factors. Endod Dent Traumatol 1996;12(6):255-64.

Kakani AK, Veeramachaneni C, Majeti C, Tummala M, Khiyani L. A review on perforation repair materials. J Clin Diagn Res 2015;9(9):09-13.

Hashem AA, Wanees Amin SA. The effect of acidity on dislodgment resistance of mineral trioxide aggregate and bioaggregate in furcation perforations: an in vitro comparative study. J Endod 2012;38(2):245-9.

Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review--Part I: chemical, physical, and antibacterial properties. J Endod 2010;36(1):16-27.

Lee SJ, Monsef M, Torabinejad M. Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations. J Endod 1993;19(11):541-4.

Yildirim T, Gençoğlu N, Firat I, Perk C, Guzel O. Histologic study of furcation perforations treated with MTA or Super EBA in dogs' teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005;100(1):120-4.

Mente J, Leo M, Panagidis D, Saure D, Pfefferle T. Treatment outcome of mineral trioxide aggregate: repair of root perforations-long-term results. J Endod 2014;40(6):790-6.

Marciano MA, Costa RM, Camilleri J, Mondelli RF, Guimarães BM, Duarte MA. Assessment of color stability of white mineral trioxide aggregate angelus and bismuth oxide in contact with tooth structure. J Endod 2014;40(8):1235-40.

Vallés M, Mercadé M, Duran-Sindreu F, Bourdelande JL, Roig M. Influence of light and oxygen on the color stability of five calcium silicate-based materials. J Endod 2013;39(4):525-8.

Parirokh M, Torabinejad M, Dummer PMH. Mineral trioxide aggregate and other bioactive endodontic cements: an updated overview - part I: vital pulp therapy. Int Endod J 2018;51(2):177-205.

Kang SH, Shin YS, Lee HS, Kim SO, Shin Y, Jung IY, et al. Color changes of teeth after treatment with various mineral trioxide aggregate-based materials: an ex vivo study. J Endod 2015;41(5):737-41.

Vanderweele RA, Schwartz SA, Beeson TJ. Effect of blood contamination on retention characteristics of MTA when mixed with different liquids. J Endod 2006;32(5):421-4.

Rahimi S, Ghasemi N, Shahi S, Lotfi M, Froughreyhani M, Milani AS, et al. Effect of blood contamination on the retention characteristics of two endodontic biomaterials in simulated furcation perforations. J Endod 2013;39(5):697-700.

Adl A, Sadat Shojaee N, Pourhatami N. Evaluation of the Dislodgement Resistance of a New Pozzolan-Based Cement (EndoSeal MTA) Compared to ProRoot MTA and Biodentine in the Presence and Absence of Blood. Scanning 2019;2019:3863069.

Aggarwal V, Singla M, Miglani S, Kohli S. Comparative evaluation of push-out bond strength of ProRoot MTA, Biodentine, and MTA Plus in furcation perforation repair. J Conserv Dent 2013;16(5):462-5.

Singla M, Verma KG, Goyal V, Jusuja P, Kakkar A, Ahuja L. Comparison of push-out bond strength of furcation perforation repair materials - glass ionomer cement type II, hydroxyapatite, mineral trioxide aggregate, and biodentine: an in vitro study. Contemp Clin Dent 2018;9(3):410-4.

Guneser MB, Akbulut MB, Eldeniz AU. Effect of various endodontic irrigants on the push-out bond strength of biodentine and conventional root perforation repair materials. J Endod 2013;39(3): 380-4.

Akcay H, Arslan H, Akcay M, Mese M, Sahin NN. Evaluation of the bond strength of root-end placed mineral trioxide aggregate and Biodentine in the absence/presence of blood contamination. Eur J Dent 2016;10(3):370-5.

Ha WN, Bentz DP, Kahler B, Walsh LJ. D90: The strongest contributor to setting time in mineral trioxide aggregate and portland cement. J Endod 2015;41(7): 1146-50.

Saghiri MA, Garcia-Godoy F, Gutmann JL, Lotfi M, Asatourian A, Ahmadi H. Push-out bond strength of a nano-modified mineral trioxide aggregate. Dental traumatology 2013;29(4):323-7.

Han L, Okiji T. Bioactivity evaluation of three calcium silicate-based endodontic materials. Int Endod J 2013; 46(9):808-14.

Han L, Okiji T. Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine. Int Endod J 2011;44(12):1081-7.

Üstün Y, Topçuo lu HS, Akpek F, Aslan T. The effect of blood contamination on dislocation resistance of different endodontic reparative materials. J Oral Sci 2015;57(3):185-90.

Gancedo-Caravia L, Garcia-Barbero E. Influence of humidity and setting time on the push-out strength of mineral trioxide aggregate obturations. J Endod 2006;32(9):894-6.

Chedella SC, Berzins DW. A differential scanning calorimetry study of the setting reaction of MTA. Int Endod J 2010;43(6):509-18.

Marquezan FK, Kopper PMP, Dullius AIdS, Ardenghi DM, Grazziotin-Soares R. Effect of blood contamination on the push-out bond strength of calcium silicate cements. Braz Dent J 2018;29:189-94.

Nekoofar MH, Davies TE, Stone D, Basturk FB, Dummer PM. Microstructure and chemical analysis of blood-contaminated mineral trioxide aggregate. Int Endod J 2011;44(11):1011-8.

Thanavibul N PA, Ratisoontorn C. Effects of blood contamination on apatite formation, pH and ion release of three calcium silicate-based materials. J Dent Assoc Thai 2019;69(3):324-33.

Kadić S, Baraba A, Miletić I, Ionescu A, Brambilla E, Ivanišević Malčić A, et al. Push-out bond strength of three different calcium silicate-based root-end filling materials after ultrasonic retrograde cavity preparation. Clin Oral Investig 2018;22(3): 1559-65.

Pane ES, Palamara JE, Messer HH. Critical evaluation of the push-out test for root canal filling materials. J Endod 2013;39(5):669-73.

Chen WP, Chen YY, Huang SH, Lin CP. Limitations of push-out test in bond strength measurement. J Endod 2013;39(2):283-7.

Deutsch AS, Musikant BL. Morphological measurements of anatomic landmarks in human maxillary and mandibular molar pulp chambers. J Endod 2004;30(6):388-90.

Alsubait SA. Effect of sodium hypochlorite on push-out bond strength of four calcium silicate-based endodontic materials when used for repairing perforations on human dentin: an in vitro evaluation. J Contemp Dent Pract 2017;18(4): 289-94.

Singh S PR, Dadu S, Kulkarni G, Vivrekar S, Babel S. An in vitro comparison of push-out bond strength of biodentine and mineral trioxide aggregate in the presence of sodium hypochlorite and chlorhexidine gluconate. Endodontology 2016;28:42-5.