The Effect of EDTA with Different Irrigation Protocols to Transforming Growth Factor-β1 Released from Root Canal Dentin
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Abstract
The Objective of this study is to evaluate effects of irrigation protocols to transforming growth factor-beta1 (TGF-β1) released from root canal dentin. Extracted human mandibular premolars (N=37) of aged 16- to 25-year-old were prepared to simulate open apex teeth. Specimens were divided into 4 experimental groups which were irrigated with one of the following protocols: group 1; 17% EDTA followed by NSS, group 2; 17% EDTA with PUI activation followed by NSS, group 3; 17% EDTA with XP-endo Finisher followed by NSS, group 4; 17% EDTA and a specimen was prepared for negative control. The amount of TGF-β1 released from root canal wall were quantified by enzyme-linked immunosorbent assay (ELISA). The data were analyzed statistically using one-way analysis of variance and Tukey’s test (P<0.05). Results showed that the greatest amount of TGF-β1 were observed in group 4 (1,341.57±419.46 pg/ml), followed by group 2 (1010.75±728.34 pg/ml) with no significant difference. The least amount of TGF-β1 were found in group 1 (656.61±454.47 pg/ml) and group 3 (685.78±318.47 pg/ml). Under the conditions of this study, it was concluded that the most effective irrigation protocol that enhanced TGF-β1 released from root canal dentin in regenerative endodontic procedure was 17% EDTA irrigation. The latter irrigation with NSS showed a comparative efficiency to 17% EDTA irrigation when PUI activation of EDTA was used.
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บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการลงตีพิมพ์ในวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นถือเป็นลิขสิทธิ์เฉพาะของคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่น หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษร จากคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นก่อนเท่านั้น
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