Effect of Saliva Contaminated and Re-Etching Time to Shear Bond Strength of Hydrophilic Sealant: An In Vitro Study

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Salinthip Phakthawat
Porntip Phajongviriyatorn
Kemporn Kitsahawong
Anoma Rattanacharoenthum


The purpose of the study was to evaluate the effect of different saliva-contaminated (C) and re-etching time (E) on the shear bond strength of hydrophilic sealant. One hundred and eighty extracted premolars were randomly divided into 9 groups (N=20) including Group 1 – noncontaminated enamel and sealant applied according to the manufacturer’s instructions (Co), Group 2 (C5) : saliva contamination at 5 seconds , Group 3 (C5+E5): saliva contamination at 5 seconds and re-etching time at 5 seconds  , Group 4 (C5+E15), Group 5 (C5+E20), Group 6 (C30), Group 7 (C30+E5), Group 8 (C30+E15) and Group 9 (C30+E20). The sealant was bonded on all specimens, then they were thermocycled for 5,000 cycles between 5°c and 55°c. The shear bond strength was tested by a universal testing machine. The result of this study revealed that group 1 showed a significantly higher shear bond strength than those the saliva-contaminated groups (group 2, 6) (p<0.05). There was no statistically significant difference between group 2 – 5 (p>0.05). On the other hand, within-group 6 – 9, Group 6 was significantly lower than group 8 (p<0.05). It could be concluded that salivary contamination undermined (weaken) the adhesion of the hydrophilic sealant to enamel and consequently reduced bond strength. Re-etching was applied to pit and fissure, resulting in significantly higher strength values of shear bond. Therefore, Re-etching on saliva-contaminated pit and fissure should be done to provide the best adhesion.


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Phakthawat S, Phajongviriyatorn P, Kitsahawong K, Rattanacharoenthum A. Effect of Saliva Contaminated and Re-Etching Time to Shear Bond Strength of Hydrophilic Sealant: An In Vitro Study. Khon Kaen Dent J [Internet]. 2021 Mar. 28 [cited 2023 Jan. 29];24(1):15-27. Available from: https://he01.tci-thaijo.org/index.php/KDJ/article/view/244097
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