Biocompatibility of Glass Ionomer Cement Containing Monocalcium Silicate Compound on Human Osteoblast

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Published: Dec 28, 2018
Keywords:
Glass ionomer cement containing monocalcium silicate Biocompatibility Osteoblast MTA

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Pornpimol Thanapornpun
Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University, Bangkok.
Punnama Siriphannon
Department of Chemistry, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok.
Jaruma Sakdee
Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University, Bangkok.

Abstract

Monocalcium silicate is one of calcium silicate based materials that have bioactive properties and influence cellular biologic response. Many studies attempted to improve biological property of glass ionomer cement by adding Monocalcium silicate. The purpose of this in vitro study was to evaluate biocompatibility, biomineralization capacity, and cell morphology of human alveolar osteoblast on glass ionomer cement containing monocalcium silicate cement or GI-CS. Method: Human osteoblast derived from human alveolar bone were used. The biocompatibility of glass ionomer cement containing 50% w/w monocalcium silicate was examine the cell viability and proliferation by MTT assay. The optical density was measured by a spectrophotometer at 570 nm. The cell morphology was investigated by Scanning electron microscope. Biomineralization assay was tested by Alizarin red staining. Result: GI-CS increased cell proliferation significantly at 1, 3 and 7 days (P<0.05). In day 3 and day 7, Cell viability of GI-CS was significantly higher than that of MTA (P < 0.05). The scanning electron microscope revealed cell spreading on both GI-CS and MTA. And both materials had biomineralization capacity. Conclusions: GI-CS and MTA showed good biocompatibility on human osteoblast.

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1.
Thanapornpun P, Siriphannon P, Sakdee J. Biocompatibility of Glass Ionomer Cement Containing Monocalcium Silicate Compound on Human Osteoblast. Khon Kaen Dent J [Internet]. 2018 Dec. 28 [cited 2024 Nov. 20];21(2):143-5. Available from: https://he01.tci-thaijo.org/index.php/KDJ/article/view/163675
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Vol. 21 No. 2 (2018)
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Articles

บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการลงตีพิมพ์ในวิทยาสารทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นถือเป็นลิขสิทธิ์เฉพาะของคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่น หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษร จากคณะทันตแพทยศาสตร์ มหาวิทยาลัยขอนแก่นก่อนเท่านั้น

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