Study of the antibacterial activity of the Tri-Kasorn-Mas remedy extracted using percolation technique

Authors

  • Suwimol Thariwong Lecturer, Division of Integration of Health, Aesthetics and Spa, Faculty of Science and Technology, Pibulsongkram Rajabhat University
  • Rodsarin Yamprasert Lecturer, Division of Applied Thai Traditional Medicine, Faculty of Public Health, Naresuan University
  • Nobpawan Watcharaput Lecturer, Division of Applied Thai Traditional Medicine, Faculty of Public Health, Naresuan University
  • Pawarisa Noppakao Lecturer, Division of Applied Thai Traditional Medicine, Faculty of Public Health, Naresuan University
  • Pitak Yoomee Lecturer, Division of Chemistry, Faculty of Science and Technology, Pibulsongkram Rajabhat University
  • Chitsirin Konkong Lecturer, Division of Integration of Health, Aesthetics and Spa, Faculty of Science and Technology, Pibulsongkram Rajabhat University
  • Atthaphol Rodkaew Lecturer, Division of Integration of Health, Aesthetics and Spa, Faculty of Science and Technology, Pibulsongkram Rajabhat University
  • Tindanai Asunee Scientist, Faculty of Public Health, Naresuan University
  • Warunya Woradulayapinij Researcher, Drug Discovery and Development Center, Office of Advanced Science and Technology, Thammasat University
  • Anusara Sihanat Lecturer, Division of Applied Thai Traditional Medicine, Faculty of Public Health, Naresuan University

Keywords:

Tri-Kasorn-Mas remedy, Acute diarrhea, Antibacterial activity, Percolation technique

Abstract

     The “Tri-Kasorn-Mas” has properties of stimulating appetite, nourishing the body’s elements, balancing internal systems, strengthening the body, and relieving diarrhea. It consists of Jatropha multifida L. (Fin-Ton), Nelumbo nucifera Gaertn. (Bua-Luang) and Aegle marmelos (L.) (Ma-Toom). Literature review reveals that the Tri-Kasorn-Mas herbal has strong antioxidant properties, and the individual herbs in the formulation also show significant antibacterial effects. However, past studies commonly used 95.0% ethanol maceration, which does not correspond with traditional Thai medicine preparation, where it involves infusing the powdered herbs in hot water. Therefore, the objective of this study was to examine the antibacterial activity against seven standard strains, both Gram-positive and Gramnegative, including: 1) Staphylococcus aureus, 2) Bacillus cereus, 3) Escherichia coli, 4) Salmonella ser. typhi
5) Salmonella typhimurium, 6) Shigella sonnei, and 7) Enterococcus faecalis. The extract was obtained through percolation with water solvent, a technique that simulates the traditional herbal infusion method.
     The results showed that the extraction yield of the Tri-Kasorn-Mas formula was 18.6 ± 0.2%. The extract exhibited antibacterial activity against three bacterial strains, with the strongest inhibition against Staphylococcus aureus, followed by Bacillus cereus and Enterococcus faecalis. This study supports traditional Thai medicine knowledge and provides a foundation for further development of the
Tri-Kasorn-Mas remedy as a treatment for acute diarrhea.

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Published

2025-12-28

How to Cite

Thariwong, S., Yamprasert, R., Watcharaput, N. ., Noppakao, P., Yoomee, P. ., Konkong, C., Rodkaew, A., Asunee, T., Woradulayapinij, W., & Sihanat, A. (2025). Study of the antibacterial activity of the Tri-Kasorn-Mas remedy extracted using percolation technique. Journal of Public Health and Health Sciences Research, 7(3), 76–87. retrieved from https://he01.tci-thaijo.org/index.php/JPHSR/article/view/277420

Issue

Vol. 7 No. 3 (2025): Vol.7 No.3 September-December 2025

Section

Research Articles

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