Antioxidant Capability and Inhibitory Effect of Leaf Extracts of Albizia lebbeck (L.) Benth. Against Formation of Mutagen and Mutagenesis
Keywords:
Albizia lebbeck (L.) Benth, Antioxidant, Ames test, Antimutagenicity, Formation of mutagen, NitroaminopyreneAbstract
Albizia lebbeck (L.) Benth. contains several pharmacological properties and has potential health benefits. In Thailand, this plant is called Ta-kuk, the young leaves of which are eaten by local people and commonly prepared by boiling. Since data are scarce on the antiformation of mutagens and antimutagenic activities of these young edible leaves.This study investigated the effect of different extracts obtained from edible leaf of A. lebbeck on the reducing the formation of mutagens, and antimutagenic activity by the Ames test using Salmonella typhimurium strains TA98 and TA 100 models without metabolic enzyme activation. Nitroaminopyrene (0.037 µg/plate) was used as a standard mutagen in our Ames test model. A. lebbeck leaves were extracted by ethanol, mix solvents, hexane, and water. Results showed that extracts exhibited strong antioxidant activities and were high in total flavonoid and total phenolic contents. In the Ames test, results showed that each extract had no toxic or mutagenic effects at various concentrations (375 - 6000 µg/plate) for both strains. These extracts could inhibit the formation of mutagens at moderate to strong activity, while A. lebbeck leaf aqueous water extract showed none to moderate capability. Moreover, all extracts showed a strong potential antimutagenicity for both TA 98 and TA 100, with criteria percentage inhibition at over 60%. Overall, these findings demonstrate that edible young leaves of A. lebbeck have antioxidant properties that could prevent the formation of mutagens and antimutagenicity against nitroaminopyrene direct-acting mutagenesis.
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