Antioxidant Potential of Ethanol Extract from Orange Fleshed Sweet Potato (Ipomoea batatas) in Murine Macrophage RAW264.7 Cell Line
Keywords:
Orange fleshed sweet potato, Antioxidant, Murine macrophage cellAbstract
Imbalance between pro-oxidant and antioxidant homeostasis is a vital risk factor for development of several chronic diseases. Orange fleshed sweet potato (OFSP) is an excellent source of beta-carotene, which has been demonstrated antioxidant potential. The present study evaluated the antioxidant effect of ethanol extract from steamed OFSP in murine macrophage cell line (RAW264.7 cells). RAW264.7 cell monolayers were pretreated with 0.5-2.0 mg/mL ethanol extract from steamed OFSP for 1 h prior to co-incubation with or without LPS for another 24 h. Cell lysate were collected to determine reactive oxygen species (ROS), glutathione (GSH), heme oxygenase-1 (HO-1) and malondialdehyde (MDA), respectively. The extract contained significant content of beta-carotene, total phenolic and flavonoids. The results indicated that the steamed OFSP extract significantly decreased LPS-induced ROS production in RAW264.7 cells without cytotoxicity. The extract also decreased MDA content but enhanced GSH content and HO-1 expression in RAW264.7 cells. The present data provided that bioactive compounds in steamed OFSP have antioxidant potential. It may be useful as an alternative plant food to reduce risk factor of oxidative stress-related chronic diseases.
References
1. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: How are they linked? Free Radic Biol Med. 2010; 49(11): 1603-16.
2. Inoue M, Sato EF, Nishikawa M, Park AM, Kira Y, Imada I, et al. Mitochondrial generation of reactive oxygen species and its role in aerobic life. Curr Med Chem. 2003; 10(23): 2495-505.
3. Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94(2): 329-54.
4. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007; 39(1): 44-84.
5. Francisqueti FV, Chiaverini LC, Santos KC, Minatel IO, Ronchi CB, Ferron AJ, et al. The role of oxidative stress on the pathophysiology of metabolic syndrome. Rev Assoc Med Bras. 2017; 63(1): 85-91.
6. Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, et al. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med. 2002; 113(9, Supplement 2): 71-88.
7. Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol. 2006; 71(10): 1397-421.
8. Wang S, Nie S, Zhu F. Chemical constituents and health effects of sweet potato. Food Res Int. 2016; 89, Part 1: 90-116.
9. Alam MK, Rana ZH, Islam SN. Comparison of the Proximate Composition, Total Carotenoids and Total Polyphenol Content of Nine Orange-Fleshed Sweet Potato Varieties Grown in Bangladesh. Foods. 2016; 5(3).
10. Islam SN, Nusrat T, Begum P, Ahsan M. Carotenoids and -carotene in orange fleshed sweet potato: A possible solution to vitamin A deficiency. Food Chem. 2016; 199: 628-31.
11. Fiedor J, Burda K. Potential role of carotenoids as antioxidants in human health and disease. Nutrients. 2014; 6(2): 466-88.
12. Aksak Karamese S, Toktay E, Unal D, Selli J, Karamese M, Malkoc I. The protective effects of beta-carotene against ischemia/reperfusion injury in rat ovarian tissue. Acta Histochem. 2015; 117(8): 790-7.
13. Mazhari NJ, Mandal AK, Thusoo TK. Carcinogenic effect of nicotine on normal mammary ductal epithelial cells and the protective role of beta-carotene. Indian J Pathol Microbiol. 2003; 46(1): 24-7.
14. Failla ML, Thakkar SK, Kim JY. In vitro bioaccessibility of beta-carotene in orange fleshed sweet potato (Ipomoea batatas, Lam.). J Agric Food Chem. 2009; 57(22): 10922-7.
15. Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed. 2013; 3(8): 623-7.
16. Prommuak C, De-Eknamkul W, Shotipruk A. Extraction of flavonoids and carotenoids from Thai silk waste and antioxidant activity of extracts. Sep Purif Technol. 2008; 62(2): 444-8.
17. Srinivasan M, Sudheer AR, Menon VP. Ferulic Acid: therapeutic potential through its antioxidant property. J Clin Biochem Nutr. 2007; 40(2): 92-100.
18. Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc. 2006; 1(3): 1112-6.
19. de Souza LF, Barreto F, da Silva EG, Andrades ME, Guimarães ELM, Behr GA, et al. Regulation of LPS stimulated ROS production in peritoneal macrophages from alloxan-induced diabetic rats: Involvement of high glucose and PPARγ. Life Sci. 2007; 81(2): 153-9.
20. Du Y, Esfandi R, Willmore WG, Tsopmo A. Antioxidant Activity of Oat Proteins Derived Peptides in Stressed Hepatic HepG2 Cells. Antioxidants (Basel). 2016; 5(4).
21. Chomchan R, Siripongvutikorn S, Maliyam P, Saibandith B, Puttarak P. Protective Effect of Selenium-Enriched Ricegrass Juice against Cadmium-Induced Toxicity and DNA Damage in HEK293 Kidney Cells. Foods. 2018; 7(6).
22. Rahman I, Kode A, Biswas SK. Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nat Protoc. 2006; 1(6): 3159-65.
23. Vandeputte C, Guizon I, Genestie-Denis I, Vannier B, Lorenzon G. A microtiter plate assay for total glutathione and glutathione disulfide contents in cultured/isolated cells: performance study of a new miniaturized protocol. Cell Biol Toxicol. 1994; 10(5-6): 415-21.
24. Tuntipopipat S, Muangnoi C, Chingsuwanrote P, Parengam M, Chantravisut P, Charoenkiatkul S, et al. Anti-inflammatory activities of red curry paste extract on lipopolysaccharide-activated murine macrophage cell line. Nutrition. 2011; 27(4): 479-87.
25. Uchida K. Role of reactive aldehyde in cardiovascular diseases. Free Radic Biol Med. 2000; 28(12): 1685-96.
26. Chung HT, Pae HO, Cha YN. Role of heme oxygenase-1 in vascular disease. Curr Pharm Des. 2008; 14(5): 422-8.
27. Tang Y, Cai W, Xu B. Profiles of phenolics, carotenoids and antioxidative capacities of thermal processed white, yellow, orange and purple sweet potatoes grown in Guilin, China. Food Sci Hum Wellness. 2015; 4(3): 123-32.
28. Bengtsson A, Namutebi A, Alminger ML, Svanberg U. Effects of various traditional processing methods on the all-trans-β-carotene content of orange-fleshed sweet potato. J Food Compost Anal. 2008; 21(2): 134-43.
29. Huang Y-C, Chang Y-H, Shao Y-Y. Effects of genotype and treatment on the antioxidant activity of sweet potato in Taiwan. Food Chem. 2006; 98(3): 529-38.
30. Rautenbach F, Faber M, Laurie S, Laurie R. Antioxidant Capacity and Antioxidant Content in Roots of 4 Sweetpotato Varieties. J Food Sci. 2010; 75(5): C400-C5.
31. Gurmu F, Hussein S, Laing M. The potential of orange-fleshed sweet potato to prevent vitamin A deficiency in Africa. Int J Vitam Nutr Res. 2014; 84(1-2): 65-78.
32. van Jaarsveld PJ, Faber M, Tanumihardjo SA, Nestel P, Lombard CJ, Benade AJ. Beta-carotene-rich orange-fleshed sweet potato improves the vitamin A status of primary school children assessed with the modified-relative-dose-response test. Am J Clin Nutr. 2005; 81(5): 1080-7.
33. Kaulmann A, Bohn T. Carotenoids, inflammation, and oxidative stress—implications of cellular signaling pathways and relation to chronic disease prevention. Nutr Res. 2014; 34(11): 907-29.
34. Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review. J Funct Foods. 2015; 18: 820-97.
35. Raza H, John A, Shafarin J. NAC attenuates LPS-induced toxicity in aspirin-sensitized mouse macrophages via suppression of oxidative stress and mitochondrial dysfunction. PLoS One. 2014; 9(7): e103379.
36. Grace MH, Yousef GG, Gustafson SJ, Truong VD, Yencho GC, Lila MA. Phytochemical changes in phenolics, anthocyanins, ascorbic acid, and carotenoids associated with sweetpotato storage and impacts on bioactive properties. Food Chem. 2014; 145: 717-24.
37. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010; 4(8): 118-26.
38. Hooshmand S, Kumar A, Zhang JY, Johnson SA, Chai SC, Arjmandi BH. Evidence for anti-inflammatory and antioxidative properties of dried plum polyphenols in macrophage RAW 264.7 cells. Food Funct. 2015; 6(5): 1719-25.
39. Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J. 2016; 15(1): 71.
40. Circu ML, Aw TY. Glutathione and apoptosis. Free Radic Res. 2008; 42(8): 689-706.
41. Bai SK, Lee SJ, Na HJ, Ha KS, Han JA, Lee H, et al. beta-Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulated macrophages by suppressing redox-based NF-kappaB activation. Exp Mol Med. 2005; 37(4): 323-34.
42. Kawata A, Murakami Y, Suzuki S, Fujisawa S. Anti-inflammatory Activity of beta-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species. In Vivo. 2018; 32(2): 255-64.
43. Kasperczyk S, Dobrakowski M, Kasperczyk J, Ostalowska A, Zalejska-Fiolka J, Birkner E. Beta-carotene reduces oxidative stress, improves glutathione metabolism and modifies antioxidant defense systems in lead-exposed workers. Toxicol Appl Pharmacol. 2014; 280(1): 36-41.
44. Hadad N, Levy R. The synergistic anti-inflammatory effects of lycopene, lutein, -carotene, and carnosic acid combinations via redox-based inhibition of NF-B signaling. Free Radic Biol Med. 2012; 53(7): 1381-91.
2. Inoue M, Sato EF, Nishikawa M, Park AM, Kira Y, Imada I, et al. Mitochondrial generation of reactive oxygen species and its role in aerobic life. Curr Med Chem. 2003; 10(23): 2495-505.
3. Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94(2): 329-54.
4. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007; 39(1): 44-84.
5. Francisqueti FV, Chiaverini LC, Santos KC, Minatel IO, Ronchi CB, Ferron AJ, et al. The role of oxidative stress on the pathophysiology of metabolic syndrome. Rev Assoc Med Bras. 2017; 63(1): 85-91.
6. Kris-Etherton PM, Hecker KD, Bonanome A, Coval SM, Binkoski AE, Hilpert KF, et al. Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. Am J Med. 2002; 113(9, Supplement 2): 71-88.
7. Aggarwal BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharmacol. 2006; 71(10): 1397-421.
8. Wang S, Nie S, Zhu F. Chemical constituents and health effects of sweet potato. Food Res Int. 2016; 89, Part 1: 90-116.
9. Alam MK, Rana ZH, Islam SN. Comparison of the Proximate Composition, Total Carotenoids and Total Polyphenol Content of Nine Orange-Fleshed Sweet Potato Varieties Grown in Bangladesh. Foods. 2016; 5(3).
10. Islam SN, Nusrat T, Begum P, Ahsan M. Carotenoids and -carotene in orange fleshed sweet potato: A possible solution to vitamin A deficiency. Food Chem. 2016; 199: 628-31.
11. Fiedor J, Burda K. Potential role of carotenoids as antioxidants in human health and disease. Nutrients. 2014; 6(2): 466-88.
12. Aksak Karamese S, Toktay E, Unal D, Selli J, Karamese M, Malkoc I. The protective effects of beta-carotene against ischemia/reperfusion injury in rat ovarian tissue. Acta Histochem. 2015; 117(8): 790-7.
13. Mazhari NJ, Mandal AK, Thusoo TK. Carcinogenic effect of nicotine on normal mammary ductal epithelial cells and the protective role of beta-carotene. Indian J Pathol Microbiol. 2003; 46(1): 24-7.
14. Failla ML, Thakkar SK, Kim JY. In vitro bioaccessibility of beta-carotene in orange fleshed sweet potato (Ipomoea batatas, Lam.). J Agric Food Chem. 2009; 57(22): 10922-7.
15. Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed. 2013; 3(8): 623-7.
16. Prommuak C, De-Eknamkul W, Shotipruk A. Extraction of flavonoids and carotenoids from Thai silk waste and antioxidant activity of extracts. Sep Purif Technol. 2008; 62(2): 444-8.
17. Srinivasan M, Sudheer AR, Menon VP. Ferulic Acid: therapeutic potential through its antioxidant property. J Clin Biochem Nutr. 2007; 40(2): 92-100.
18. Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc. 2006; 1(3): 1112-6.
19. de Souza LF, Barreto F, da Silva EG, Andrades ME, Guimarães ELM, Behr GA, et al. Regulation of LPS stimulated ROS production in peritoneal macrophages from alloxan-induced diabetic rats: Involvement of high glucose and PPARγ. Life Sci. 2007; 81(2): 153-9.
20. Du Y, Esfandi R, Willmore WG, Tsopmo A. Antioxidant Activity of Oat Proteins Derived Peptides in Stressed Hepatic HepG2 Cells. Antioxidants (Basel). 2016; 5(4).
21. Chomchan R, Siripongvutikorn S, Maliyam P, Saibandith B, Puttarak P. Protective Effect of Selenium-Enriched Ricegrass Juice against Cadmium-Induced Toxicity and DNA Damage in HEK293 Kidney Cells. Foods. 2018; 7(6).
22. Rahman I, Kode A, Biswas SK. Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nat Protoc. 2006; 1(6): 3159-65.
23. Vandeputte C, Guizon I, Genestie-Denis I, Vannier B, Lorenzon G. A microtiter plate assay for total glutathione and glutathione disulfide contents in cultured/isolated cells: performance study of a new miniaturized protocol. Cell Biol Toxicol. 1994; 10(5-6): 415-21.
24. Tuntipopipat S, Muangnoi C, Chingsuwanrote P, Parengam M, Chantravisut P, Charoenkiatkul S, et al. Anti-inflammatory activities of red curry paste extract on lipopolysaccharide-activated murine macrophage cell line. Nutrition. 2011; 27(4): 479-87.
25. Uchida K. Role of reactive aldehyde in cardiovascular diseases. Free Radic Biol Med. 2000; 28(12): 1685-96.
26. Chung HT, Pae HO, Cha YN. Role of heme oxygenase-1 in vascular disease. Curr Pharm Des. 2008; 14(5): 422-8.
27. Tang Y, Cai W, Xu B. Profiles of phenolics, carotenoids and antioxidative capacities of thermal processed white, yellow, orange and purple sweet potatoes grown in Guilin, China. Food Sci Hum Wellness. 2015; 4(3): 123-32.
28. Bengtsson A, Namutebi A, Alminger ML, Svanberg U. Effects of various traditional processing methods on the all-trans-β-carotene content of orange-fleshed sweet potato. J Food Compost Anal. 2008; 21(2): 134-43.
29. Huang Y-C, Chang Y-H, Shao Y-Y. Effects of genotype and treatment on the antioxidant activity of sweet potato in Taiwan. Food Chem. 2006; 98(3): 529-38.
30. Rautenbach F, Faber M, Laurie S, Laurie R. Antioxidant Capacity and Antioxidant Content in Roots of 4 Sweetpotato Varieties. J Food Sci. 2010; 75(5): C400-C5.
31. Gurmu F, Hussein S, Laing M. The potential of orange-fleshed sweet potato to prevent vitamin A deficiency in Africa. Int J Vitam Nutr Res. 2014; 84(1-2): 65-78.
32. van Jaarsveld PJ, Faber M, Tanumihardjo SA, Nestel P, Lombard CJ, Benade AJ. Beta-carotene-rich orange-fleshed sweet potato improves the vitamin A status of primary school children assessed with the modified-relative-dose-response test. Am J Clin Nutr. 2005; 81(5): 1080-7.
33. Kaulmann A, Bohn T. Carotenoids, inflammation, and oxidative stress—implications of cellular signaling pathways and relation to chronic disease prevention. Nutr Res. 2014; 34(11): 907-29.
34. Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review. J Funct Foods. 2015; 18: 820-97.
35. Raza H, John A, Shafarin J. NAC attenuates LPS-induced toxicity in aspirin-sensitized mouse macrophages via suppression of oxidative stress and mitochondrial dysfunction. PLoS One. 2014; 9(7): e103379.
36. Grace MH, Yousef GG, Gustafson SJ, Truong VD, Yencho GC, Lila MA. Phytochemical changes in phenolics, anthocyanins, ascorbic acid, and carotenoids associated with sweetpotato storage and impacts on bioactive properties. Food Chem. 2014; 145: 717-24.
37. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010; 4(8): 118-26.
38. Hooshmand S, Kumar A, Zhang JY, Johnson SA, Chai SC, Arjmandi BH. Evidence for anti-inflammatory and antioxidative properties of dried plum polyphenols in macrophage RAW 264.7 cells. Food Funct. 2015; 6(5): 1719-25.
39. Kurutas EB. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr J. 2016; 15(1): 71.
40. Circu ML, Aw TY. Glutathione and apoptosis. Free Radic Res. 2008; 42(8): 689-706.
41. Bai SK, Lee SJ, Na HJ, Ha KS, Han JA, Lee H, et al. beta-Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulated macrophages by suppressing redox-based NF-kappaB activation. Exp Mol Med. 2005; 37(4): 323-34.
42. Kawata A, Murakami Y, Suzuki S, Fujisawa S. Anti-inflammatory Activity of beta-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species. In Vivo. 2018; 32(2): 255-64.
43. Kasperczyk S, Dobrakowski M, Kasperczyk J, Ostalowska A, Zalejska-Fiolka J, Birkner E. Beta-carotene reduces oxidative stress, improves glutathione metabolism and modifies antioxidant defense systems in lead-exposed workers. Toxicol Appl Pharmacol. 2014; 280(1): 36-41.
44. Hadad N, Levy R. The synergistic anti-inflammatory effects of lycopene, lutein, -carotene, and carnosic acid combinations via redox-based inhibition of NF-B signaling. Free Radic Biol Med. 2012; 53(7): 1381-91.
Downloads
Published
2019-03-22
How to Cite
Saokosol, Y., Praengam, K., Sukprasansap, M., & Tuntipopipat, S. (2019). Antioxidant Potential of Ethanol Extract from Orange Fleshed Sweet Potato (Ipomoea batatas) in Murine Macrophage RAW264.7 Cell Line. Journal of Nutrition Association of Thailand (Online), 54(1), 37–54. Retrieved from https://he01.tci-thaijo.org/index.php/JNAT/article/view/171225
Issue
Section
Research article
License
Upon acceptance of an article, copyright is belonging to the Nutrition Association of Thailand.
