Comparison of Cord Blood Processing Procedure using Standard and Automated Centrifugation Techniques

บทคัดย่อ

Abstract:

The National Cord Blood Bank of Thailand has been set up since May 2002. Collection and
processing  of cord blood donations have been performed to serve for patients waiting for unrelated,
autologous  and related stem cell transplants. Prior to the addition of cryoprotectant, the reduction of
the  volume of cord blood units is needed in order to allow lower storage and overall expenses. This
retrospective  study was undertaken to evaluate cord blood processing procedure using standard and
automated  centrifugation techniques. Altogether, 388 cord blood units, which were cryopreserved at
the  National Blood Centre, Thai Red Cross Society from May 2002 to December 2006 were studied. Of
these,  255 and 133 cord b loodu nitsw erec ollectedf romn ormald eliverya ndc esareans ection. A c omparison
 of total white blood cell (WBC) and total CD34+ cells in cord blood that collected from normal
delivery  were similar to cesarean section (p > 0.05). Total red blood cells (RBC), hematocrit and volume
of cord blood that collected from cesarean section were significantly higher than those collected from
normal  delivery (p < 0.05). Moreover, using a standard centrifugation technique the collection efficiency
of WBC (% WBC recovery) is better than an automated technique (p < 0.001), while CD34+ cells after
processing  is not different (p > 0.05). On the other hand, using and automated technique, % RBC
depletion  is more effective than standard centrifugation technique (p < 0.001). This may be due to the
addition  of hydroxyethyl starch during processing with an automated technique. Inconclusion, CD34+
cells  of cord blood processing using standard centrifugation technique were similar to automated centrifugation
 technique. Although an automated centrifugation technique needs more expense, it allows
lower  storage and long-term liquid nitrogen supplied. The automated centrifugation technique appears
to  be suitable in cord blood processing in view of large-scale National Cord Blood Bank.