Comparison of Low Ionic Strength Solution, Polyethylene Glycol and Manual Polybrene for Antibody Detection
คำสำคัญ:
Antibody screening LISS PEG MPบทคัดย่อ
Abstract :
The sensitivity of the antibody detection technique is important in providing compatible
blood. In cases that a pattern of weak reactions fails to indicate specificity, or the presence of an
antibody is suspected but cannot be demonstrated, the use of enhancing reagents may be helpful.
Objectives : The purpose of this study was to compare low ionic strength solution (LISS), polyethylene
glycol (PEG) and manual polybrene (MP) techniques for enhancing antibody detection in indirect antiglobulin
testing (IAT). Material and methods : Antibody screening was performed in 200 unknown
donor blood samples in parallel. The antibody screening was also performed in 122 samples of known
antibody specificities, of which these samples gave positive results by all techniques. The antibody
titration was performed on these samples to compare the sensitivity of the assays. Results: The results
were evaluated by Wilcoxon Signed Ranks test. The positive results of antibody screening performed
in the 200 unknown samples, using LISS-IAT, PEG-IAT and MP-IAT techniques, were found in 5(2.5%),
5(2.5%) and 4(2%) samples, respectively. The results evaluated by Friedman test showed no significant
difference among LISS, PEG and MP techniques (P>0.05). In addition, titration studies were performed
on 101 samples of known antibody specificities. Comparison of mean scores of the three techniques
demonstrated that LISS-IAT (15.6) gave a higher mean score in detecting anti-Lea and anti-Leb than the
MP-IAT (11.2) and the PEG-IAT (10.1), respectively. However, the PEG-IAT showed a higher mean
scores in detecting anti-D, anti-E, anti-Jka, anti-Jkb, anti-M and anti-N than the MP-IAT and the LISSIAT,
respectively. Furthermore, PEG-IAT had a higher mean score in detecting anti-Fya, anti-Fyb, anti-
S, anti-Mia and anti-H than LISS-IAT and MP-IAT, respectively. Conclusion : The PEG-IAT is the best
in all technique systems for antibody detection. But the LISS-IAT is the better technique for antibody
detection in Lewis system.
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