Comparison of Apheresis Platelets Leukocytes Reduced Prepared with Trima Accel and Haemonetics MCS Plus Cell Separators

บทคัดย่อ

Abstract:

Objective: The purpose of this research is to study the performance of a new automated blood cell separator
Trima  Accel (Gambro BCT Inc., Lakewood, CO, USA) compared with the MCS Plus (Haemonetics, Braintree, MA,
USA), t hata reu sedf orr outinea pheresisp lateletsl eukocytesr educed. M aterialsa ndM ethods:P lateletpheresisw as
collected  from the same donor using the same group of 30 donors for each machine. The target platelet yields were
set  at 4.0x 1011 platelets. Following the procedures, plateletpheresis data were compared in terms of platelet yields,
collection  efficiency (CE), processing times and residual leukocyte counts. The study was carried out at
Hemapheresis  Unit, the National Blood Centre, Thai Red Cross Society, during January 17, 2006 to May 30, 2006.
Platelets  were counted with an automated blood cell counter (Coulter Counter T-890, Coulter Electronics, England)and
residual leukocytes were counted with flow cytometry (FACScan, Becton Dickinson, USA). Results: In this study,
comparison of plateletpheresis characteristics revealed that platelet yield was significantly higher for Trima Accel
thanfor MCS Plus (p = 0.003) and the CE of Trima Accel was higher than MCS Plus (p = 0.06). Additionally, Trima
Accel  had significantly (p < 0.001) shorter processing times by an average of 15 minutes than MCS Plus. Regarding
leukoreduction,  all plateletpheresis units obtained from both machines had fewer than 1x106 white blood cells except
one  unit prepared by Trima Accel. No severe reactions occured during and after procedure for both studies. Conclusion:
 Trima Accel processes more efficiency and faster than MCS Plus. Apheresis platelets leukocytes reduced
complies well with current US and European standards.