Study on the Centrifugation Speed that Affect Swirling Phenomenon in Platelet Concentrates by Using Heraeus Cryofuge 8500i

บทคัดย่อ

Abstract :

Objective : This study aimed to investigate the speed in heavy spin for Platelet Rich Plasma (PRP) for preparing Platelet Concentrates (PC) in order to decrease discarded PC caused by swirling phenomenon. Material and Method : Various centrifugation speeds for PRP for preparing PC were set as; 3,920 rpm (5,102g), 3,600 rpm (4,303g), 3,500 rpm (4,068g), 3,400 rpm (3,838g), 3,300 rpm (3,616g) and 3,200 rpm (3,400g), at 22 + 2°C for 4 minutes, with acceleration of 9 and deceleration of 4 by using refrigerated centrifuge (Heraeus Cryofuge 8500i, Thermo Scientific, Germany). After centrifugation, the amount of cells in Fresh Plasma (FP) and PC were assessed using volume testing, platelet count, leukocyte content, and swirling test. Results : The results showed that platelet count in PC were decreased while platelet count in FP were increased when centrifugation speed decreased. On the second day of PC storage, the better score of swirling phenomenon was found on unit preparing by decreasing speed. The percentage of swirling phenomenon score 5+ at 3,920 rpm was 94.44, 3,600 rpm was 97.22, 3,500 rpm was 98.61, 3,400 rpm was 98.61, 3,300 rpm was 100, and 3,200 rpm was 100. Conclusion : This study was found that the optimum speed for separating PRP for preparing PC is 3,500 rpm at 22 + 2°C for 4 minutes, with acceleration of 9 and deceleration of 4 by using Heraeus Cryofuge 8500i giving plate count in PC of about 92.32 + 21.86 x109 (% passed = 95.83), % passed of swirling phenomenon = 100, % swirling phenomenon score 5+ = 98.6 (71/72 units), FP volume in limit, and % passed of FP = 100.