A Comparative Study between the Automated Complete Blood Count (CBC) and the Manually-Determined Hematocrit Level and the Cell Count Estimated from Peripheral Blood Smear
Keywords:
Peripheral blood smear, CBC, Aumated, Hematocrit, WBC, PlateletAbstract
Abstract : Objectives : To determine the relationship between the automated complete blood count and the manually-determined hematocrit level and direct estimation of the amount of white blood cell and platelet from peripheral blood smear. To determine the effect of hematocrit level in esting the amount of platelet count from peripheral blood smear. Design : Descriptive study.
Materials and Methods : Penipheral blood samples were taken from 115 patients at the Hematology Clinic, Department of Medicine, Phramongkutklao Hosipital After smearing on two glass sides, the blood samples were preserved in EDTA tubes and sent for automated complete blood count using a Coulter Stks machine. Manual hematocrit determination was carried out by hilling the EDTA-pre-served blood into a hematocrit tube and reading the value after centifugation. We estimated the number of white blood cell and platet from peripheral blood smear by averaging cells counted from 10 oil fields. Results : The hematocrit level determined from both methods showed a good correlation (r = 0 97). The white blood cell count obtained from both methods disclosed a poor comelation (r = = 0.58). Estimation for the number of white blood cell using peripheral blood smear is not recommended. The number of platelet count from both methods showed a good cortelation (r = 0.88). The estimated number of platelet count from penpheral blood smear was 1 per oil field equals 16,903 cells per mm3. There is a good corelation between the number of automated if the platelet count was lower than 24 per oil field (r = 091). It is also noted the hematocnt level has no effect on the estimated
platelet count. Conclusion : Manual hematocnit determination is comparable to that obtained from automated hematocrit. The platelet count estimated from peripheral blood smear is useful with no comelation with the value of hematocrit.
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