Evaluation of anti-D Reagents as Rh (D) Blood Typing
Keywords:
Anti-D, Rh (D) typingAbstract
Abstract : A total of eleven anti-D reagents, from commercial and one from the National Blood Centre, Thai Red Cross Society (NBC, TRCS) were evaluated for their efficacy as Rh/D) blood typing reagents. They were tested for potency, avidity, specicity and ability to detect weak Dantigens, as well as DVI cells. It was found that all anti-D sera were exceeded the potency and avidity requirements of WHO standard by having a titer of greater than 1:64 with Rir cells and giving positve result within suxty second of muxing in manual slide test. Most of them were capable of detecting 20 samples of weak D antigen. For the seven samples of DVI cells, two commercial anti-D sera were unable to detect any of them where as another two commercial anti-D sera detect these cells by direct agglutination. The other six commercial and the NBC TROS anti-D sera were able to detect DVI cells by indirect antigiobulin test (IAT). In conchusion, the ant-D reagents from various companies ditered in efficacy for detection of weak Dand D VI cells. Furthermore some of these cells could be typed as Rh(D) positive by one reagent and as Rh(D) negative by another reagent. Evaluation of anti-D reagent is essentual in selecting appropriate reagent that can be used for both donor and patient Rh(D) typing.
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