Stability of Coagulation Factors in Thawed and Refreezing Previously Thawed Fresh Frozen Plasma after Various Times of Storage

Abstract

Abstract : Fresh Frozen Plasma (FFP) is widely used in patients with coagulation disorders. The problem is that the FFP request may be canceled after thawing of FFP. The aim of this study was to evaluate the possibility of reusing of the previously thawed FFP. Ten units of thawed FFP were studied for Kactivity of factor II, V, VIL VII X X X as frst thawed FFP thawed 2 hour refrozen FFP and thawed 4 hour refrozen FFP at 0, 2, 4, 6, 24 hours after thawing. The result of mean Mactivity of factor VII in frst thawed FFP only at 24 hours was statistically decreased compared with 0 hour but was still within the normal range. The results of mean %activity of factor Vill in thawed 2 and 4 hour refrozen FFP at 0 hour were statically decreased compared with 0 hour after first thawed but also were still within the normal range. There were statistically decreased in mean %activity of factor Vand VIII in thawed 2 and 4 hour refrozen FFP at 2 up to 24 hours compared with 0 hour after first thawed and factor V were less than the normal range Therefore first thawed FFP which stored at 4 C may be used up to 24 hours while thawed 2 and 4 hour refroen FFP which stored at 4'C may be used immediately after thawing.