Hb E Screening Test
Keywords:
Hemoglobin E, Screening testAbstract
Abstract : Hb E screening test was modified from the microcolumn DEAE Sephadex A 50 chromatography method. It has been used for screening of Hb E cases by applying a 40 Mil. blood sample dissolved in 5 miliitres of working buffer pH 8.5 into a pasteur pipette containing DEAE Sephadex A50 of 5 centrimetres high. Hemoglobin is adsorbed on the column when the blood sample moves through. An aliquot of 5 millitres of working buffer pH 8.5 is applied in order to fix the hemobin. Then Hb E is eluted with 10 millitres of working buffer pH 8.2 and red colour along the column is noticed whereas Hb A, Hb F. Hb H and Hb Bart's remain on the upper part of the column. The white color of DEAE Sephadex A50 at the lower part of the column indicates no Hb E in the blood
sample.
The results obtained from the study on Hb E screening test are as follows :
1. Five hundred blood samples from pregnant women were tested along with cellulose acetate Hb electrophoresis method, 50 corresponding Hb E cases were shown by both methods.
2. Five hundred and five Hb cases out of 4,922 pregnant women were found, je. 10.2% incidence was shown.
3. Upon determination of Hb E proportion in 323 Hb E positive cases by Hb E screening test, the Hb proportion ranging from 11.5-94.0% of total hemoglobin can be read.
4.Only 68% of those 323 Hb E trait cases were found to be positive when determining by 2 min OF method.
5.alpha-thal.1 trait in positive Hb E cases reported by Hb E screening test was mostly found in cases of Hb E proportion ranging between 15-20%.
Hb E screening test is a simple and accurate method for Hb E screening. It can be applied in searching for Hb E carriers which are useful for the control and prevention of Hb E/Bthalassemia.
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References
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