Monoclonal Anti-C 3d Antibody (Clone 5C4)

Authors

  • Rachanee Kaewmongkol National Blood Centre, Thai Red Cross Society
  • Kanjana Aiemumporn National Blood Centre, Thai Red Cross Society
  • Suwit Phonimit National Blood Centre, Thai Red Cross Society
  • Achara Siripongsanusit National Blood Centre, Thai Red Cross Society
  • Tasanee Sakuldamrongpanich National Blood Centre, Thai Red Cross Society

Keywords:

Monoclonal anti-C3d

Abstract

Abstract: The clone 5C4 which is a stable mouse hybridoma line secteting monoclonal anti-C3d was  selected. It was high potency (titer) and specity which reacted with complement coated red red blood cells both in vitro and in vivo. Anti-C3d secreting clone 5C4 can be used at a final dilution of 1 to 300 to provide an excellent antiglobulin reagent when mixed with a suitable dilution of rabbit anti-IgG serum to prepare anti-human globulin reagent (AHS). To evaluate the potency and specificity. It was tested the potency and speciticity to compare with polyclonal (rabbit) antiglobulin reagent conventionally prepared by NBC TRC and 2 commercial reagents. It was found that the blended antiglobuin reagent gave as good quaity as polvclonal frabbiti anti globuin reagent. This report
suggested that monoclonal anti-C3d from clone 5C4 could substitute anti-C 3d from rabbit source in the routine preparation of antiglobulin reagent.

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Published

2018-12-30

Issue

Vol. 12 No. 2: April-June 2002

Section

นิพนธ์ต้นฉบับ (Original article)

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