The B weak Phenotype in a Thai Family weak

Authors

  • Ruangrong Cheepsattayakorn Department of Pathology, Faculty of Medicine, Chiang Mai University
  • Ladda Fongsatitkul Blood Bank, Faculty of Medicine, Chiang Mai University
  • Amornrat Romphruk Blood Transfusion Center, Faculty of Medicine, Khon Kaen University
  • Tanin Bhoopat Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University
  • Heinrich F. Steger Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University
  • Chanvit Leelayuwat Department of Clinical Immunology, Faculty of Associated Medical Sciences, Khon Kaen University
  • Sucheela Chomsook Blood Bank, Faculty of Medicine, Chiang Mai University

Keywords:

B weak phenotype, B allele, PCR-SSP, DNA sequencing

Abstract

Abstract: We reported two subjects with B weak phenotype in the same family. The proband was a man who donated blood at Mahaharaj Nakorn Chiang Mai Hospital. The second subject was his father. ABO blood grouping of the donor gave negative reaction with anti-A, anti-Band anti-A B (Thai Red Cross) by slide test so he was classified as group O. The cell grouping by standard tube
test and gel test showed 2+ and mixed-field agglutination (mi) with anti-B and anti-A.B (Thai Red Cross) but showed negative reaction with anti-A and two monoclonal anti-B-from DiaMed and Biosoot). Weak anti-B was found in his serum but did not react with his own red cells. Antibody screening and direct antiglobulin test were negative. His saliva contained H subtance while no B
substance was detected. ABO genotyping of the subjects was performed by several methods including polymerase chain reaction using sequence specific primers (PCR-SSP) in exon 6, 7 and intron 6; polymerase chain reaction with restriction fragment length polymorohism (PCR-PCR-RELP) in exon 6, 7 and DNA sequencing at nucleotide postion 810-1,065 in exon 7 revealed two difference alles and compatible with B, O1 genotype. Family study demonstrated that his father had the same genotype and showed B weak phenotype as the donor. His mother and his brother were blood group A. Both of them were heterozygous A'(C467T), O1. The genotype of B weak of this report was different from the previous B weak alleles which had point mutation at T863G, G871A, C873G, A1036G, G1055A or B3 allele which had C10547 in exon 7. Our B weak phenotype may have B weak allele which can not be detected by these methods. Neither history of serious ilness nor previous blood transfusion was found in the donor and his father. Serologic study of the donor 1 year later revealed that he was B weak phenotype. The B weak donor may be at risk of mistyping as blood group O. This may have adverse consequences when transfusing the B weak blood to group Orecipients so the accurate ABO blood typing is very important in transfusion medicine.

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Published

2018-12-30

Issue

Vol. 12 No. 2: April-June 2002

Section

นิพนธ์ต้นฉบับ (Original article)

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