การศึกษาเบื้องต้นเพื่อเปรียบเทียบปฏิกิริยาแอนติเจน - แอนติบอดี ของหมู่เลือดใน Polyethylene Glycol, LISS และ Saline Medium

Apapan Srisarin; Wajarin  Shuabunmee; Suphavadee Rojpipatgul; Mayurat Boonkaew; Jariya Saipin

Authors

Apapan Srisarin Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University
Wajarin Shuabunmee นักศึกษาสาขาเทคนิคการแพทย์ คณะเทคนิคการแพทย์ มหาวิทยาลัยมหิดล
Suphavadee Rojpipatgul นักศึกษาสาขาเทคนิคการแพทย์ คณะเทคนิคการแพทย์ มหาวิทยาลัยมหิดล
Mayurat Boonkaew Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University
Jariya Saipin Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

Blood group antigen-antibody reaction, Enhancement medium, Polyethylene glycol medium, LISS medium, Saline medium

Abstract

Abstract: To find an alternative problem solving for some low titer antibodies. The comparison of blood group antigen-antibody reaction in 3 different mediums: polyethylene glycol (PEG), LISS and NSS were studied. Known serum samples of 12 anti-D, 10 anti-E, 7 anti-Le, 7 anti-Le' and 5 anti-Mi' were diluted in PEG, LISS and NSS. Each of the dilutions was tested with the screening cells
suspended in the 3 mediums respectively. The scores or titer obtained from techniques using PEG medium were compared with those from the conventional methods, suitable for each type of antibodies, using LISS and NSS mediums. The results evaluated by Wilcoxon Signed Ranks test showed that PEG medium did better in enhancing antigen-antibody of anti-D and anti-E when compared
with saline medium (p < 0.05) but not different from LISS (p > 0.05). PEG also enhanced anti-Le, anti-Le when papainized cells were used. The results were also statistically significant different (p < 0.05) when compared with the couple of sera and cells reacted in LISS and saline medium. Anti-Mi was not enhanced by PEG when compared with the 2 mediums (p > 0.05). Some aspects ofsome techniques in this preliminary study might be improved in further studies.

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