The prevalence of red cell antibodies among patients and pregnant women in Pakchongnana Hospital
Keywords:
Red cell antibodies, Patients, Pregnant women, แอนติบอดีต่อเม็ดเลือดแดง, ผู้ป่วย, หญิงตั้งครรภ์Abstract
Abstract:
Background: The antibody screening test is an essential component of pretransfusion testing before blood transfusion. Antibody identification must be performed to determine antibody specificity in case of positive antibody screening. Limited data are available on the prevalence of red cell antibodies among patients and pregnant women from lower northeastern Thailand. This study aimed to determine the prevalence of red cell antibodies among patients and pregnant women in Pakchongnana Hospital. Materials and Methods: Blood samples from 601 pregnant women and 2,488 patients were tested using antibody screening by standard tube technique (STT). Positive samples were determined for antibody specificity and analyzed according to sex, age groups, ABO and Rh types. Moreover, red cell genotyping was performed in samples with positive autocontrol. Results: The prevalence of antibodies among pregnant women and patients was 1.33% and 1.89%, respectively. The frequencies of positive red cell antibodies were significantly higher among patients ≥ 40 years (p = 0.036), belonging to a single antibody (57.4%), multiple antibodies (12.8%) and unidentified antibodies (29.8%). Anti-Mia was the most common in the two groups, followed by anti-P1, anti-E, anti-Lea and anti-Leb. Additionally, for 2 patients having anti-Mia, the predicted phenotypes were confirmed to be Mi(a-) using multiplex PCR. Conclusion: This is the first report of the prevalence of red cell antibodies among patients and pregnant women in Pakchongnana Hospital, which attempted to provide red cell antigens that, have the potential for alloantibody formation. Hence, the prompt management of donor blood units corresponding to this data could be applied to ensure safer blood transfusions.
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