Antioxidant Activity and Gas Chromatography Mass Spectrometry Analysis of the Composition of Ben-Ja-Kot Recipe Extract
Keywords:
Ben-Ja-Kot, Antioxidant, Total phenolic content, Total flavonoid content, Gas Chromatography-Mass SpectrometryAbstract
Ben-Ja-Kot (BJK) recipe of Thai Traditional Medicine contains of 5 species of herb Angelica dahurica (AD), Atractylodes lancea (AL), Ligusticum sinense (LS), Angelica sinensis (AS) and Artemisia annua (AA) have been used for fever of mucus and asthma cough. The aims of this study were 1) to study total phenolic content and flavonoid content 2) to study antioxidant activity by DPPH and ABTS assay and 3) to study compounds was determined bioactive compound by GC-MS analysis of Ben-Ja-Kot (95% ethanol and aqueous) extract. The results of the research indicated that total phenolic content was showed highest in the aqueous extracts of AD (32.51±0.63 mg GAE/g extract) and ethanolic extracts was AS (47.44±0.43 mg GAE/g extract). Total flavonoid content of aqueous extracts had the highest of BJK (40.70±0.20 mg QAE/g extracts) and for ethanolic extracts was AS (99.70±0.17 mg QAE/g extract). The ability on antioxidant scavenging by DPPH was found the highest effective of aqueous was LS by IC50 value 1,149.00±41.43 µg/ml. For ethanolic extract of AS (780.17±12.04µg/ml). For scavenging ability by ABTS had the highest effect aqueous extract of LS as IC50 values 64.14±3.66 µg/ml and for ethanolic extracts was AS IC50 values 391.37±5.29 µg/ml. GC-MS analysis of aqueous and ethanolic extract were 11 and 16 compounds of AD. AL was detected 11 aqueous compounds and of 9 compounds ethanolic extracts. Aqueous of LS was found 14 compounds and 21 compounds in ethanolic extracts. AS aqueous and ethanolic were found 8 and 19 compounds. AA aqueous and ethanolic were 8 and 20 compounds. In addition, two solvent extract of Ben-Ja-Kot aqueous and ethanolic were 5 and 4 compounds, respectively. Further work is progress forwards understanding this recipes role as an antipyretic activity.
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