Screening of Lanna Medicinal Plants with Anti-inflammatory Property Assessed by Free Radical Scavenging Activities
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Abstract
Lanna is the northern eight provinces of Thailand. Several Lanna medicinal plants have potential for use by traditional practitioners for a long time without the scientific support data. Therefore, it is worthy to study the inflammatory property via free radical scavenging of such plants. Four kinds of Lanna medicinal plants, Caesalpinia sappanL., Leea rubraBlume ex Spreng., Vernomia volkameriforiaWall.ex DC., and Schleichera oleosaMerr. were extracted with ethanol by soxhletûs apparatus to give the ethanolic extracts. The extracts were studied for free radical scavenging activity by ABTS0+ decolorizing assay, scavenging effect on superoxide anion and nitric oxide. Leea rubraBlume ex Spreng. exhibited the highest ABTS 0+ scavenging activity with the Vitamin C Equivalent Antioxidant Capacity (VCEAC) = 0.6100 g L-ascorbic acid/g of extract and Trolox Equivalent Antioxidant Capacity (TEAC) = 0.9540 g trolox/g of extract. Caesalpinia sappanL. showed the highest superoxide anion scavenging activity with an EC50 value of 4.51 μg/ml, which is comparable to the activity of L-ascorbic acid (EC50 = 8.83 μg/ml). Furthermore, it exhibited the highest nitric oxide scavenging activity with EC50 value of 0.61 μg/ml. The activity was comparable to curcumin with an EC50 value of 1.05 μg/ml. The medicinal plant extracts showed a concentration dependent antiradical activity. The extract with highest activity will be further studied for use as an active ingredient in Medical Spa products or anti-inflammation products.
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