Production of Pseudojujubogenin from Callus Cultures of Bacopa monnieri (L.) Wettst.

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DOI: https://doi.org/10.14456/ijps.2007.7
Keywords:
Bacopa monnieri(L.) Wettst. pseudojujubogenin callus

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Sirilak Kamonwannasit
Faculty of Pharmaceutical Sciences, 123 Khon Kaen University ,Naimaung , Muang, Khon Kaen, Thailand 40002
Waraporn Putalun
Faculty of Pharmaceutical Sciences, 123 Khon Kaen University ,Naimaung , Muang, Khon Kaen, Thailand 40002
Watoo Phrompittayarat
Regional Medical Sciences Center, 9th Phitsanulok, Thailand 65000
Kornkanok Ingkaninan
Faculty of pharmaceutical sciences, Naresuan University, Phitsanulok, Thailand 65000
Hiroyuki Tanaka
Department of Medicinal Plant Breeding, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan

Abstract

Bacopa monnieri(L.) Wettst. (Scrophulariaceae), widely distributed in Asia, Australia and America. This plant has traditionally been used in India as an important constituent of Ayurveda to treat disorders such as epilepsy, insanity, cardiotonic and neurosis. B. monnieri was studied in vitrousing leaf and stem as explants on Murashige and Skoog (MS) medium supplemented with different of auxins and cytokinins. After four weeks cultured, 100% of callus formation was obtained from medium supplemented with 2,4-D 1 mg/l and kinetin 1 mg/l. Callus induced from stem as explants cultured in medium supplemented with kinetin 1 mg/l and 2,4-D 0.5 mg/l contained the highest level of pseudojujubogenin (9.29±1.62 mg/g dry wt). Callus cultured in medium supplemented with kinetin 0.5 mg/l and 2, 4-D 1 mg/l was obtained the highest fresh weight increase (362±165 mg/flask).

Article Details

Issue
Vol. 3 No. 1 (2007): Volume 3 No.1 January - June, 2007
Section
Pharmacy

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