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Bacopa monnieri(L.) Wettst. (Scrophulariaceae), widely distributed in Asia, Australia and America. This plant has traditionally been used in India as an important constituent of Ayurveda to treat disorders such as epilepsy, insanity, cardiotonic and neurosis. B. monnieri was studied in vitrousing leaf and stem as explants on Murashige and Skoog (MS) medium supplemented with different of auxins and cytokinins. After four weeks cultured, 100% of callus formation was obtained from medium supplemented with 2,4-D 1 mg/l and kinetin 1 mg/l. Callus induced from stem as explants cultured in medium supplemented with kinetin 1 mg/l and 2,4-D 0.5 mg/l contained the highest level of pseudojujubogenin (9.29±1.62 mg/g dry wt). Callus cultured in medium supplemented with kinetin 0.5 mg/l and 2, 4-D 1 mg/l was obtained the highest fresh weight increase (362±165 mg/flask).
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