Antioxidative Activity and Antimutagenicity of Three Plants in Annonaceae in Plant Genetics Conservation at Khok Phutaka, Amphur Phuwiang, Khon Kaen
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Abstract
This study aimed to investigate the antioxidative activity and antimutagenicity of six extracts of three plants in Annonaceae, collected from Khok Phutaka area, Amphur Phuwiang, Khon Kaen. The results showed the strong antioxidative activities of the extracts from stem-root of Ellipeiopsis cherrevensis and leaf of Polyalthia evecta with EC₅₀ at 14.3 and 14.7 µg/ml, respectively. The other extracts with relative order of activity were leaf of E. cherrevensis > leaf of P. debilis > stem-root of P. evecta > stem-root of P. debilis (EC₅₀ = 17.9, 22.8, 46.3 and 177.9 µg/ml, respectively). The plant total phenolic contents are well correlated with their antioxidative activities. For pre-incubation bacterial mutation test, the extracts from leaf and stem-root of E. cherrevensis showed mutagenic effect on Salmonella typhimurium TA98 and TA100 in the absence of S-9 mix. Whereas in the presence of S-9 mix, the mutagenicity was found in the extracts from leaf of E. cherrevensis, leaf and stem-root of P. debilis and leaf of P. evecta. The antimutagenicity was detected in both TA98 and TA100 in all extracts in the presence of S-9 mix. In contrast, in the absence of S-9 mix only the extracts from stem-root and leaf of P. debilis; and leaf of P. evecta showed the antimutagenicity to TA98. Therefore, these three plants are interesting to be further studied on their anticancer activity.
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References
Ames BN. 1972. A bacterial system for detecting mutagens and carcinogens. In: Mutagen effects of environmental contaminants. H.E. Sutton and M.I. Harris (Eds.), Academic Press. NY, pp. 57-66.
Araki A, Marumatsu M, Matsushima T. 1984. Comparison of mutagenicities of N-nitrosamines on Salmonella typhimuriumTA100 and Escherichia coli wp2 uvr A/pkm 101 using rat and hamster liver S9. Gann 75: 8-16.
Aruoma OI., Halliwell B, Williamson G. 1997. In vitromethods for characterizing potential prooxidant and antioxidant actions of nonnutritive substances in plant foods. In: O.I. Aruoma and S.L. Cuppett (Eds.) Antioxidant methodology. AOCS Press, USA. pp. 173-204.
Derbré S, Gil S, Taverna M, Boursier C, Nicholas V, Demey-Thomas E, Vinh J, Susin SA, Hocquemiller R, Poupon E. 2008. Highly cytotoxic and neurotoxic acetogenins of the Annonaceae: New putative biological targets of squamocindetectedbyactivity-based protein profiling. Bioorg Med Chem Lett18(21): 5741-5744.
Kanokmedhakul S, Kanokmedhakul K, Ohtani JJ, Isobe M. 1998. A diynoic acid from Polyalthiaevecta. Phytochemistry 47: 131-133.
Kanokmedhakul S, Kanokmedhakul K, Yodbuddee D, Phonkerd N. 2003. New antimalarial bis-dehydroaphorphine alkaloids from Polyalthia debilis. J Nat Prod 66(5): 616-619.
Kanokmedhakul S, Kanokmedhakul K, Kantikeaw I, Phonkerd N. 2006. 2-substituted furans from the roots of Polyalthia evecta. J Nat Prod 69(1): 68-72.
Kijjoa A, Bessa J, Pinto MMM, Anatachoke C, Silva AMS, Eaton G, Herz W. 2002. Polyoxygenated cyclohexenes derivatives from Ellipeiopsis cherrevensis. Phytochemistry59: 543-549.
Kushi LH, Meyer KA, Jacobs DR. 1999. Cereals, legumes, and chronic disease risk reduction: evidence from epidemiologic studies. Am J Clin Nutr70: 451S- 458S.
Makkar HPS, Bluemmel M, Borowy NK, Becker K. 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods. J Sci Food Agri61: 161–165.
Matsushima T, Sawamura M, Hara K, Sugima T. 1976. A safe substitute for polychlorinated biphenyls as an inducer of metabolic activation system. In: In vitro metabolic activation in mutagenesis testing. F.J. de Serres, J.R. Fouts, J.R. Bend and R.M. Philpot (Eds.), Amsterdam: Elsevier 1 North Holland. pp. 85-88.
Ravikuman YS, Mahadevan KM, Kumaraswamy MN, Vaidya VP, Manjunatha H, Kumar V, Satyanarayana ND. 2008.Antioxidant, cytotoxic and evaluation of alcohol extract of Polyalthia cerasoides (Roxb.) Bedd. Env Toxicol Pharmacol 26: 142-146.
Samitinanta T. 2001. Flora of Thailand. Botanic Garden, Ministry of Forrest.
Sripanidkulchai B, Tattawasart U, Laupatarakasem P, Vinitketkumneun U, Sripanidkulchai K, Furihata C, Matsushima T. 2002. Antimutagenic and anticarcinogenic effects of Phyllanthus amarus. Phytomedicine 9: 26-32.
Woisky RG, Salatino A. 1998. Analysis of propolis: some parameters and procedures for chemical quality control. J Agri Res 37: 99-105.