Antioxidant activity and total phenolics of herbal extract of combination from mulberry leaves, turmeric rhizome, lakoocha’s wood and shiitake.

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Wanwisa Boonsinchai
Suthasinee Thapphasaraphong
Natsajee Nualkaew
Somsak Nualkaew


Introduction: The  aimed of study to determine and compare antioxidant activity and total phenolics of  herbal extracts combinationfrom 4 plantsincluding mulberry leaves, turmeric rhizome, lakoocha's wood and shiitake. Methods: Antioxidant activity testing methods by DPPH  assay and total phenolic compound were performed. Results: From antioxidant activity by DPPH assay, Lakoocha's wood showed the best activity withIC50 was 18.32 ± 0.005 μg / ml and TPC was 2,493.32 ± 0.260 mg GAE / g sample. In the mixture of 2 plant extracts from mixture A (mulberry leaves and turmeric rhizome) showed the best antioxidant  by DPPH assay which IC50 was 0.076 ± 0.007 μg/ml. The concentration of each plant in mixture A was 2.6 times lower thaneach single extracts and TPC of mixture A was 445,635.39 ± 0.008 mg GAE/g sample which is higher than TPC of single mulberry leaves extract and turmeric rhizome extract. The study of the relation with the Pearson’s Correlation between TPC and antioxidant activity showed statistically significant (p-value <0.001). Conclusion: This study showed that using herbal extracts combination could provide higher antioxidant activity than single plant extract.  The combination of the suitable plants and optimized concentration could make the best synergistic effects.  Therefore, using plant extract combination should be more advantage for the development natural health product in the near future.


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Pharmaceutical Sciences


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