Bioaccessibility of Aflatoxin B₁ in Grinded Corn and Peanut by in vitro Digestion Model
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Abstract
Aflatoxin B₁ (AFB₁) is a secondary metabolite produced principally by Aspergillus flavus and Aspergillus Parasiticus. This toxin represents a significant health hazard for those exposed to it continually for a prolonged period. Aflatoxigenic molds are commonly found in corn and peanuts, and under certain conditions aflatoxins will be formed. In vitro digestion is a method that has been used to estimate the bioaccessibility of chemicals and contaminants in foods. An in vitro digestion experiment was carried out in this study to analyze the bioaccessibility of AFB₁ in grinded corn and peanut. Trifluoroacetic acid-derivatized AFB₁ was measured using a HPLC-fluorescent detector. Results indicated that the bioaccessibility of AFB₁ in grinded corn and peanut was relatively high at 95% 94%, respectively. When AFB₁-contaminated grinded corn and peanut are ingested, most of the AFB₁ will be dissolved into chyme, with this strongly influencing the bioavailability of AFB₁ in humans. This may have important implications on dietary exposure. Findings from the current study could be applied to improve risk assessment of ingested AFB₁.
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References
Chan DY, Black WD, Hale†BA. Cadmium bioavailability and bioaccessibility as determined by in vitro digestion, dialysis and intestinal epithelial monolayers, and compared to in vivo data. J Envi Sci Health A Tox Hazard Subst EnviEng 2007; 42(9): 1283-1291.
Dziki UG, Dziki D, Baraniak B, Lin R. The effect of simulated digestion in vitro on bioactivity of wheat bread with TBF (Tartary Buckwheat Flavones) addition, LWT - Food Science and Technology 2009; 42(1): 137-143.
Liu CS, Glahn RP, Liu RH. Assessment of carotenoid bioavailability of whole foods using a Caco-2cell culture model coupled with an in vitrodigestion. J Agric Food Chem 2004; 52(13):4330-7.
Oomen AG, Rompelberg CJ, Bruil MA, Dobbe CJ, Pereboom DP, Sips AJ. Development of an invitro digestion model for estimating the bioaccessibility of soil contaminants. Arch Envi Contam Toxicol 2003; 44(3): 281-287.
Oomen AG, Rompelberg CJ, Van de kamp E, Pereboom DP, De Zwart LL, Sips AJ. Effect of bile type on the bioaccessibility of soil contaminants in an in vitro digestion model. Arch Envi Contam Toxicol 2004; 46(2):183-188.
Schroder L, Basta NT, Casteel SW, Evans TJ, Payton ME, Si J. Validation of the In Vitro Gastrointestinal (IVG) Method to Estimate Relative Bioavailable Lead in Contaminated Soils. J Envi Qual 2004; 33(2): 513-521.
Versantvoort CH, Oomen AG, Van de kamp E, Rompelberg CJ, Sips AJ. Applicability of an invitro digestion model in assessing thebioaccessibility of mycotoxins from food. Food Chem Toxicol 2005; 43(1): 31-40.
Wittsiepe J, Schrey P, Hack A, Selenka F, Wilhelm M. Comparison of different digestive tract models for estimating bioaccessibility of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) from red slag “Kieselrot” Int J Hyg Envi Health 2001;203(3): 263-273.
Xing GH, Yang Y, Chan JKY, Tao S, Wong MH. Bioaccessibility of polychlorinated biphenyls indifferent foods using an in vitro digestion method. Enviro. Pollut 2008; 156(3): 1218-1226.