Antioxidant Activities of The Soybean Extracts Obtained by Classical Extraction

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DOI: https://doi.org/10.14456/ijps.2010.27
Keywords:
soybean extracts daidzein genistein classical method antioxidant activities

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Ratana Indranupakorn
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, HuachiewChalermprakiet University.
Parapat Sobharaksha
Pharmaceutical Technology Department, Faculty of Pharmaceutical Science, Silpakorn University.
Manee Luangtana-anan
Pharmaceutical Technology Department, Faculty of Pharmaceutical Science, Silpakorn University.

Abstract

Many Thai people consume soybean extracts obtained by classical boiled water extraction daily.
However, there is no scientific data on their composition of phytonutrients. The present study aims to assess the antioxidant power and composition of isoflavones in soybean extracts from classical extraction. HPLC analyses of the extracts showed daidzein and genistein as the major components. The antioxidant activities of the soybean extracts were evaluated by free radical scavenging (DPPH and ABTS) assays. Total phenolic and isoflavone contents were also determined by using Folin-Ciocalteau reagent and developed HPLC methods, respectively. The IC50 values of daidzein, genistein and reference standard, trolox, in DPPH radical scavenging assay were 0.41, 0.39 and 0.28 mg/ml, respectively. Daidzein and genistein were also found to scavenge the ABTS+ with IC50 values of 0.55 and 0.53 mg/ml, respectively, and the activities were higher than trolox (IC50 = 0.88 mg/ml). Measurement of total phenolic contents of the soybean extracts showed that daidzein and genistein account for about half part of total phenolics in soybean extracts. These results obtained in this study clearly indicate that the soybean extracts obtained by classical extraction has a significant potential to use as a natural antioxidant agent.

Article Details

Issue
Vol. 6 No. 3 (2010): Volume 6 No.3 September - December, 2010
Section
Pharmaceutical Sciences

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