Preparation and stability study of mouth care product from stinglessQs bee propolis extract
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Abstract
Introduction: The aim of this study was to formulate month care product containing certainly amount of stinglessos bee propolis extract with appropriate stability. Methods: Stinglessos bee propolis was macerated in ethanol and removed excess wax using hexane. The amount of alpha-mangostin in extract was analyzed using high performance liquid chromatography. The antioxidant activity was perform by diphenylpicryhydrazyl scavengingassay. The formulations of mouthwashes and toothpastes were prepared with various concentration of stinglessos bee propolis extract. The stability of formulation was investigate in 30 ºC 60% RH (normal condition) and 45 ºC 75% RH (accelerated condition) for 6 months. The physical appearance, pH, label amount (%) and antioxidant activity were evaluated. Results: Form the formulation, the maximum amount of the extract in mouthwash and toothpaste were 0.05% w/w and0.5% w/w, respectively. The formulation of mouth care product contained the extract exhibited yellow color. The pH of mouthwash was in the range of 5.87t6.15 and decrease statistically significant to the range of 4.60 U 5.01 in 6months. The label amount (%) decreased from the range of 101 U 102.9% to 85.4 t 93.3% when kept in accelerated condition for 6 months. The antioxidant activity of mouthwash still maintained within 6 months which showed the inhibition (%) in the range of 39 U 79.5%. The toothpaste exhibited appropriate stability in both condition. The pH of tooth paste was in the range of 7.62 t 7.93. The viscosity of toothpaste was in the range of 8113 U 9125cPs. The toothpaste had label amount (%) in the range of 97.2 U 103.3%. The IC50 of toothpaste was in the range of 56.32 t 531.13 mg/ml. Conclusion:The formulation of month care products containing certainly amount of stinglessos bee propolis extract were successfully prepared. The toothpaste showed appropriate stability. However, the stability of mouthwash should be improved by adding the buffering agent. The antibacterial activity of mouthwash and toothpaste will be investigate in nearly future.
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