Influence of Duration Times of Storage on the Caffeic Acid Contents in Pandanus amaryllifolius Root Decoction
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Abstract
Introduction: Pandanus amaryllifolius Roxb. Is a medicinal plant used in the treatment of diabetes in Thai Traditional Hospital Sakon Nakhon Vernerable Luang Pu Fab Subhatto. Decoction of this plant root is inovention for patients to prepare the medication in this formulated. The present study aimed to investigate the effects of duration times of storage 1, 24, 72 and 120 hours after preparation on the amount of caffeic acid (CA), in P. amaryllifolius root. Methods: using the Thin layer Chromatography (TLC) and the High Performance Thin Layer Chromatography (HPTLC) Techniques Results: CA in the sample showed Rf value of 0.62 and its contents inter sample after keep the preparation at 1, 24, 72 and 120 hours were 3.04±0.03 6.33±0.01, 11.44±0.02 and 12.77±0.01 percentage respectively, storage of the sample at 120 h. gave the highest content of CA (P<0.05) Conclusion: Duration times of storage of P. amaryllifolius root. Decoction afforded CA content. This investigation provides important information and reference about storage time of decoction cautionary P.amaryllifolius root.
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