Study of bioactive compound and their bioactivities from Langkhae (Baccaurea macrocarpa (Miq.) Müll. Arg) peel extract for application of natural product

Abstract

Introduction: Langkhae (Baccaurea macrocarpa (Miq.) Müll. Arg) is a plant belonging to family Euphorbiaceae. It is a fruit in the southern of Thailand and can high yield 300-500 Kg/tree. This research aimed to compare of phytochemical screening, bioactive compound and antioxidants activity of Langkhae peel extract. Methods: The phytochemical screening was analyzed by colourimetric methods. The total phenolic content of the crude extracts was measured by Folin- Ciocalteu method and the antioxidant activity was measured by DPPH, ABTS and FRAP assays. Results: The percentage of yield of 95% methanol crude extract of B. macrocarpa showed the highest yield (12.33%). The test determination of Flavonoids contents found that an 95% ethanol crude extract is the highest (0.14±0.05 mg GAE/g sample). The condensed tannins contents of 50% ethanol crude extract is the highest (0.54±0.07 mg GAE/g sample). The hydrolysable tannins found that 95% methanol crude extract is the highest (0.60±0.02 mg GAE/g sample). The phenolic compound found that and 95% methanol crude extract is 2.13±0.21 mg GAE/g sample. The antioxidant activity of three methods found that 95% methanol crude extract is the highest. Conclusion: Fortunately, these results suggest the potential of several parts from Langkhae peel extract as medicine or cosmetic agent free radical.