Dicentrine production in cell suspensions and hairy root cultures of Stephania suberosa

Main Article Content

Tharita Kitisripanya
Jukrapun Komaikul
Waraporn Putalun


Introduction: Stephania suberosa Forman is the herbal climber plants with very large tuber. Dicentrine, aporphine alkaloids, is the bioactive compound that was found in tuberous root of S. suberosa with various pharmacological activities like acetylcholinesterase inhibition, antitumor effect and antiarrhythmic effect. However, S. suberosa tuber take a long time to growth therefore plant tissue culture technique is the optional approach for bioactive secondary metabolites production from S. suberosa with shorter than natural cultivated period and might be apply to the industrial scale production. Materials and Methods: In this study, S. suberosa cell suspensions were induced and hairy root cultures were established by Agrobacterium rhizogenes. Both cultures were elicited by yeast extract and methyl jasmonate to investigate their effect on dicentrine production. Results: After subculture into fresh media, approximately one month, cell suspensions and hairy root cultures of S. suberosa produced dicentrine average range 0.2 – 1.5 and 4.5 – 8.9 mg/g dry weight, respectively. In cell suspensions, yeast extract and methyl jasmonate did not improve dicentrine production except yeast extract at concentration 0.1 mg/ml in day 9 which increased dicentrine more 1.2 time than control group. To compare with the control group in hairy root cultures of S. suberosa, yeast extract did not significantly affected dicentrine content but methyl jasmonate suppressed dicentrine production. Conclusion: To obtain the high dicentrine production, hairy root cultures of S. suberosa was suitable than cell suspension cultures. And elicitation with yeast extract only increase dicentrine in cell suspensions of S. suberosa while methyl jasmonate suppressed dicentrine production in both cell suspensions and hairy root cultures of S. suberosa.


Download data is not yet available.

Article Details



Chaichana N, Dheeranupattana S, Jatisatienr A, et al. Response of stemona alkaloid production in Stemona sp. to chitosan and yeast extract elicitors. Curr Res J Biol Sci2012; 4: 449-45 4.

Cho HY, Lee-Parsons CWT, Yoon SYH, Rhee HS, Park JM. Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Biotechnol Lett2007; 29: 2001–2005.

Cho HY, Rhee HS, Yoon SYH, Park JM. Differential induction of protein expression and benzophenanthridine alkaloid accumulation in Eschscholtzia californica suspension cultures by methyl jasmonate and yeast extract. J Microbiol Biotechn 2008; 18: 255–262.

El-Sayed M, Verpoorte R. Effect of phytohormones on growth and alkaloid accumulation by a Catharanthus roseus cell suspension cultures fed with alkaloid precursors tryptamine and loganin. J Plant Biotechnol 2002; 68: 265-270.

Eskandari Samet A, Piri KH, Kayhanfar M, Hasanloo T. Influence of jasmonic acids, yeast extract and salicylic acid on growth and accumulation of hyosciamine and scopolamine in hairy root cultures of Atropa belladonna L. Intl J Agric: Res & Rev 2012; 2: 403-409.

Frick S, Kutchan TM. Molecular cloning and functional expression of O-methyltransferases common to isoquinoline alkaloid and phenylpropanoid biosynthesis. Plant J 1999; 17: 329–339.

Ingkaninan K, Temkitthawon P, Chuenchom K. et al. Screening for acetylcholinesterase inhibitory activity in plants used in Thai traditional rejuvenating and neurotonic remedies. J Ethnopharmacol 2003; 89: 261-264.

Memelink J, Verpoorte R, Kijne JW. ORCAnization of jasmonate responsive gene expression in alkaloid metabolism. TRENDS Plant Sci 2001; 6: 212-219

Santisuk T, Chayamarit K, Pooma R, Suddee S. Thailand Red Data: Plants, ONEP Biodiversity Series Vol. 17. Bangkok: Integrated promotion technology Co., Ltd. 2006.

Sriprang S, Khorana N, Ingkaninan K. Acetylcholinesterase inhibitor from Stephania suberosa Forman. Naresuan Univ Sci J 2006; 3: 1-11.

Stevigny C, Bailly C, Quentin-Leclercq J. Cytotoxic and antitumor potentialities of aporphinoid alkaloids. Curr Med Chem 2005 5: 173-182.

Woo SH, Sun NJ, Cassady JM, Snapka RM. Topoisomerase II inhibition by aporphine alkaloids. Biochem Pharmacol 1999; 57: 1141-1145.

Young ML, Su MJ, Wu MH, Chen CC. The electrophysiological effects of dicentrine on the conduction system of rabbit heart. Brit J Pharmacol 1994; 113: 69-76.

Zayed R, Wink M. Induction of tropane alkaloid formation in transformed root cultures of Brugmansia suaveolens (Solanaceae). Z Naturforsch 2004; 59c: 863-867.

Ziegler J, Facchini PJ, Geißler R, et al. Review: Evolution of morphine biosynthesis in opium poppy. Phytochem 2009; 70: 1696-1707.