Study of Some Antioxidant to the Amout of Quercetin in Marum Extract.

Main Article Content

Supawadee Daodee
Thanakom Panya
Narin Chansri
Bungorn Sripanidkulchai


Introduction: Moringa (Moringa oleifera Lam.) is one of the multipurpose plants that can be used as vegetable and medicinal plants. It is known as Marum in Thai. This plant is reported to contain some compounds such as alkaloids, flavonoids and cinnamates. Quercetin is one of the flavonoid constituents shown in the leaves of this plant and showed anti-inflammation activity. Therefore, this research aimed to study the effect of some antioxidant to the amount of quercetin in Moringa oleifera leaves. Materials and Method: This study focused on the effect of some antioxidant to the analysis of quercetin. So, Butylated hydroxyanisole (BHA) in various amounts was added into the process of determination. The amount of quercetin was then analyzed by HPLC method.  Results: The crude extract of Moringa oleifera leaves was prepared by ethanol extraction and used in this study. It was found that butylated hydroxyanisole added into the reaction could increase the amount of quercetin when analyzed by HPLC (11.24 mg per g extract). Conclusion: Thus, it could be concluded that the analysis of quercetin in Moringa Oleifera leaves should add some of the antioxidant such butylated  hydroxyanisole  in the process of analysis. This could confirm the actual amount found in the leave of this plant and promote the accurate and reliable results to use in the other studies.


Download data is not yet available.

Article Details



Babu SC. Rural nutrition interventions with indigenous plant foods: a case study of vitamin deficiency in Malawi International Food Policy Research Institute, Washington, DC. Biotech Agronomy Soc Environ 2000; 4(3): 169-179.
Bharali R, Tabassum J, Azad MH Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolizing enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pacific Journal of Cancer Prevention. 2003; 4: 131-139.
Caceres A, Cabrera O, Morales O, Mollinedo P, P Mendia. Pharmacological properties of Moringa oleifera. 1: Preliminary screening for antimicrobial activity. J of Ethnopharmaco 1991; 33: 213-216.
Caceres A, Saravia A, Rizzo S, Zabala L, De Leon E, Nave F. Pharmacologic properties of Moringaoleifera. 2: Screening for antispasmodic, antiinflam-matory and diuretic activity. J of Ethnopharmaco. 1992; 36: 233-237.
Chuang PH, Lee CW, Chou JY, Murugan M, Shieh BJ, Chen HM. Anti- fungal activity of crude extracts and essential oil of Moringa oleifera Lam. Bioresource Technology 2007; 9 8(1): 232-236.
Fahey JW. Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, andprophylactic properties (part 1). Tree for life J. 2005 [cited 2012 August 20]. Available from: http: //
Fuglie LJ. The miracle tree: Moringa oleifera: Natural nutrition for the tropics. Training Manual. 2001. Church World Service, Dakar, Senegal.
Goyal BR, Agrawal BB, Goyal RK, Mehta AA. Phytopharmacology of Moringa oleifera Lam.-an overview. Indian J Nat Prod Resour 2007; 6(4): 347 -353.
Hertog MGL, Hollman PCH and Venema DP. Optimization of a quantitative HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits. J of Agricultural and Food Chem 1992; 40: 1591-1598.
Kumar NA, Pari L. Antioxidant action of Moringa oleifera Lam. (drumstick) against anti tubercular drug induced lipid peroxidation in rats. J Med Food2003; 6(3): 255-259.
Lako J, Trenerry C, Wahlqvist ML, Wattanapenpaiboon N, Subramanium S, Premier R.Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods. 03088146Food Chem 2007; 101(4): 1727–1741.
Lamson DW, Brignall MS. Antioxidants and cancer III: Quercetin. Altern Med Rev 2000; 5(3): 196-208.
Mehta K, Balaraman R, Amin AH, Bafna PA, Gulati OD J. Effect of fruits of Moringa oleifera on thelipid profile of normal and hypercholesterolaemic rabbits. Ethnopharmacol 2003; 86(2-3):191-195.
Nuutila AM, Kammiovirta K, Oksman-Caldentey KM. Comparison of methods for the hydrolysis of flavonoids and phenolic acids from onion and spinach for HPLC analysis. Food Chem 2002; 76: 519–525.
Pari L, Kumar NA. Hepatoprotective activity of Moringa oleifera on antitubercular drug-induced liverdamage in rats.J Med Food 2002; 5(3):171-177.
Punya T, Chansri N, Sripanidkulchai B, et al. Effect of antioxidant to the analysis of quercetin in Moringa oleifera Leaves. Proceedings of the Fourth International Conference on Natural Products for Health and Beauty, 2012 Nov 28-30;
Chiang Mai, Thailand. Ramachandran C, Peter KV, Gopalakrishnan PK. Drumstick (Moringa oleifera): a multipurpose Indian vegetables. Economic Botany 1980; 34(3): 276 -283.
Rahman MM, Sheikh MI, Sharman SA, Islam MS, Rahman MA, Rahman MM, Alam MF. Antibacterial activity of leaf juice and extracts of Moringa oleifera Lam against some human pathogenic bacteria. CMU J Nat Sci 2009; 8(2): 219-227.
Sreelatha S, Padma PR. Antioxidant Activity and Total Phenolic Content of Moringa oleifera Leaves in Two Stages of Maturity. Plant Foods Hum Nutr 2009; 64:303–311.
Vieira GH, Moura JA, Angelo AM, Costa RA, Vieira RH. Antibacterial effect (in vitro) of Moringa oleiferaand Annona muricata against gram positive and gram negative bacteria. Rev Inst Med Trosao Paulo 2010; 52(3): 129-132.
Williamson G, Plumb GW, Uda Y, Price KR, Rhodes MJ. Dietary quercetin glycosides: antioxidant activity and induction of the anticarcinogenic phase II marker enzyme quinine reductase in Hepalclc 7 cells. Carcinogenesis 1996; 17(11): 2385-2387