Study of Some Antioxidant to the Amout of Quercetin in Marum Extract.

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Supawadee Daodee
Thanakom Panya
Narin Chansri
Bungorn Sripanidkulchai

Abstract

Introduction: Moringa (Moringa oleifera Lam.) is one of the multipurpose plants that can be used as vegetable and medicinal plants. It is known as Marum in Thai. This plant is reported to contain some compounds such as alkaloids, flavonoids and cinnamates. Quercetin is one of the flavonoid constituents shown in the leaves of this plant and showed anti-inflammation activity. Therefore, this research aimed to study the effect of some antioxidant to the amount of quercetin in Moringa oleifera leaves. Materials and Method: This study focused on the effect of some antioxidant to the analysis of quercetin. So, Butylated hydroxyanisole (BHA) in various amounts was added into the process of determination. The amount of quercetin was then analyzed by HPLC method.  Results: The crude extract of Moringa oleifera leaves was prepared by ethanol extraction and used in this study. It was found that butylated hydroxyanisole added into the reaction could increase the amount of quercetin when analyzed by HPLC (11.24 mg per g extract). Conclusion: Thus, it could be concluded that the analysis of quercetin in Moringa Oleifera leaves should add some of the antioxidant such butylated  hydroxyanisole  in the process of analysis. This could confirm the actual amount found in the leave of this plant and promote the accurate and reliable results to use in the other studies.

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