Phenolic and flavonoids contents and antibacterial activity of ethanolic extract of propolis.
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Abstract
The aim of this work was to study properties of Thai propolis extract (TPE) for the development of dental pulp capping material. Raw propolis materials were collected from Nong Khai province and extracted using 95% ethanol. Total flavonoid contents were determined by Aluminum chloride colorimetric method and 2,4-Dinitrophenylhydrazine colorimeteric method. Total phenolic compounds were determined using Folin-Ciocalteau colorimetric method. The results showed that TPE had flavonoids 10% (w/w of TPE) and phenolic 2.95% (w/w of TPE). TPE had an inhibition effect against both Streptococcus mutans and Lactobacillus casei. The minimum inhibitory concentration of TPE was 22.5 mg/ml and 11.25 mg/ml for Streptococcus mutans and Lactobacillus casei, respectively. The minimum bactericidal concentration of TPE was 45 mg/ml for both bacteria. TPE concentration 90 mg/ml (two-fold of minimum bactericidal concentration which planned for pharmaceutical preparation) had no cytotoxicity on human dental pulp cells at 6, 12, 24 and 72 hours. The results of this study suggest that TPE is suitable for development of dental pulp capping material because it had bactericidal effect against Streptococcus mutans and Lactobacillus casei which were often found bacteria in infected human dental pulp and it had no cytotoxicity on human dental pulp cells.
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